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Cat. No.: MCA-5 (for 5mg)

Cat. No.: MCA-25 (for 25mg)

Alternative Names 

[MCA]/[Lys(Dnp)]-CoV Main Protease (Mpro) Substrate; [MCA]/[Lys(Dnp)] Mpro Substrate; [MCA]/[Lys(Dnp)] 3CLpro Substrate; CoV 3CL Protease Fluorogenic Substrate (MCA/Dnp); CoV Main Protease Fluorescent Substrate (MCA/Dnp); CoV 3CL Protease Fluorescent Substrate (MCA/Dnp)

Description 

MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a substrate for coronavirus Mpro/3CLpro, based on fluorescence resonance energy transfer (FRET). After cleavage, this substrate releases the fluorescent group MCA (methylcoumarin), with a maximum excitation wavelength of 320 nm and maximum emission wavelength of 405 nm. By measuring fluorescence, Mpro activity can be detected. Since the COVID-19 pandemic at the end of 2019, this substrate has been widely used in screening kits for Mpro/3CLpro inhibitors or in detecting kits for Mpro/3CLpro protease activity of the novel coronavirus (2019-nCoV).

Purity 

>95%

Molecular Weight 

1514.7

Sequence 

MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2

Source 

Synthetic

Formulation 

5 mg dissolved in 165 µl DMSO, or 30.28 mg dissolved in 1 ml DMSO to prepare a 20 mM solution. This product is provided in a 20 mM concentration using DMSO.

Storage Conditions

Store in a dark place at -20℃. The product is valid for one year.

Precautions

• If using small amounts or using frequently, consider aliquoting and storing at -20℃ in the dark. Repeated freeze-thaw cycles can affect the product's performance.
• This product is intended for scientific research use by professionals only and must not be used for clinical diagnostics or treatments, food or drug applications, and must not be stored in a regular household.
• For your safety and health, please wear a lab coat and disposable gloves while handling.

Related:

• MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 (Coronavirus Main Protease Fluorogenic Substrate)
• Dabcyl-KTSAVLQSGFRKME-Edans (Coronavirus Main Protease Fluorogenic Substrate)
• MCA-YVADAPK(Dnp)-OH (ACE2 Fluorogenic Substrate)
• MCA-APK(Dnp)-OH (ACE2 Fluorogenic Substrate)
• MCA-EIDLMVLDK-Dnp (BACE1 Fluorogenic Substrate)

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Only for research and not intended for treatment of humans or animals

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