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Cat. No.: MCAA-5 (for 5mg)

Cat. No.: MCAA-25 (for 25mg)

Alternative Names 

MCA-Ala-Pro-Lys(Dnp)-OH; ACE2 substrate

Description 

MCA-APK(Dnp)-OH is a fluorogenic substrate for carboxypeptidase ACE2, based on fluorescence resonance energy transfer (FRET). After cleavage, this substrate releases the fluorescent group MCA (7-methoxycoumarin-4-yl)acetyl, with a maximum excitation wavelength of 320 nm and maximum emission wavelength of 405 nm. By measuring fluorescence, the activity of ACE2 can be detected. Since the COVID-19 pandemic at the end of 2019, this substrate has been widely used in screening kits for ACE2 inhibitors or fluorescent detection kits for ACE2 activity. Unlike MCA-YVADAPK(Dnp)-OH (P9735), this substrate is not cleaved by proteases such as Caspase-1, BMP-1/PCP, and endopeptidase Meprin alpha Subunit/MEP1A, offering better specificity. MCA: (7-Methoxycoumarin-4-yl)acetyl, Dnp: 2, 4-Dinitrophenyl.

Purity 

>95%

Molecular Weight 

696.7

Sequence 

MCA-Ala-Pro-Lys(Dnp)-OH

Source 

Synthetic

Formulation 

5 mg dissolved in 359 µl DMSO, or 13.93 mg dissolved in 1 ml DMSO to prepare a 20 mM solution. This product is provided in a 20 mM concentration using DMSO.

Storage Conditions

Store in a dark place at -20℃. The product is valid for one year.

Precautions

• If using small amounts or using frequently, consider aliquoting and storing at -20℃ in the dark. Repeated freeze-thaw cycles can affect the product's performance.
• This product is intended for scientific research use by professionals only and must not be used for clinical diagnostics or treatments, food or drug applications, and must not be stored in a regular household.
• For your safety and health, please wear a lab coat and disposable gloves while handling.

Related:

• MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 (Coronavirus Main Protease Fluorogenic Substrate)
• Dabcyl-KTSAVLQSGFRKME-Edans (Coronavirus Main Protease Fluorogenic Substrate)
• MCA-YVADAPK(Dnp)-OH (ACE2 Fluorogenic Substrate)
• MCA-APK(Dnp)-OH (ACE2 Fluorogenic Substrate)
• MCA-EIDLMVLDK-Dnp (BACE1 Fluorogenic Substrate)

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