All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: LCRSK-50 (for 50T)

Cat. No.: LCRSK-200 (for 200T)

Description

Long Circular RNA Synthesis Kit catalyzes the ligation of ion-molecule substrates, including single-stranded DNA (ssDNA) and single-stranded RNA (ssRNA), to form circular molecules. In the circularization reaction, it is necessary for the substrate ssDNA/RNA to possess a 5'-phosphate group and a 3'-OH group. This kit is capable of producing single-stranded DNA templates for rolling circle amplification or rolling circle transcription experiments, as well as generating circular RNAs ranging from 15 to 500 nucleotides in length for next-generation sequencing.

Feature

• This kit can convert over 85% of single-stranded RNAs with 5'-phosphate and 3'-hydroxyl groups into circular RNAs within 60 minutes.
• The kit is capable of producing single-stranded DNA templates for rolling circle amplification or rolling circle transcription experiments and generating circular RNAs ranging from 15 to 500 nucleotides for next-generation sequencing.

Quality Control

• Free from RNase and DNase contamination.
• Purity >90% as determined by Coomassie Brilliant Blue staining of SDS-polyacrylamide gel electrophoresis.

Storage

The minimum shelf life is 1 year at -20°C.

Note

• When using this kit, please wear laboratory coat, disposable latex gloves, disposable masks, and utilize RNase-free consumables to avoid RNase contamination.
• Due to RNA handling, strict adherence to RNA handling protocols is necessary to avoid RNase contamination. Reagents and consumables should be treated with DEPC to remove RNase or ensured to be RNase-free. As single-stranded RNA is involved, consider adding an appropriate amount of RNase Inhibitor, with a recommended final concentration of 1~2U/µL.
• Substrates for ligation must be phosphorylated.
• For circularization of ssDNA, such as oligodeoxynucleotides or cDNA, add MnCl2 to a final concentration of 2.5mM.
• When using urea-denaturing polyacrylamide gel or agarose gel electrophoresis, use pre-cooled electrophoresis buffer or conduct electrophoresis in an ice bath to prevent RNA degradation caused by high electrophoresis temperatures.

Related: Circular RNA Synthesis Kit, Circular RNA Synthesis

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory