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Cat. No.: Lb5Cas12a-100 (for 100pmol)

Cat. No.: Lb5Cas12a-1k (for 1000pmol)

Description

Lb5Cas12a (also known as Lb5Cpf1) is a DNA endonuclease guided solely by crRNA, originating from the Lachnospiraceae bacterium ND2008 strain. In the presence of a PAM (TTTN) sequence in the target double-stranded DNA, Lb5Cas12a can selectively cleave the target DNA, causing a break in the DNA double-strand and generating sticky ends. Notably, Lb5Cas12a specifically cleaves single-stranded DNA targets without relying on a PAM sequence.

Furthermore, both double-stranded and single-stranded DNA targets can activate the trans-cleavage activity of Lb5Cas12a (also known as collateral cleavage activity). When the Lb5Cas12a enzyme forms a ternary complex with crRNA and target DNA, it is activated to perform trans-cleavage activity against non-specific sequences of single-stranded DNA, resulting in the cleavage of any sequence of single-stranded DNA in the system. Therefore, Lb5Cas12a enzyme can be utilized not only for specific cleavage of dsDNA in vitro but also for the rapid detection of target nucleic acids.

Storage