All products have special prices for bulk purchase, please contact us for more details if required.

Cat. No.: LAMEX-1k (for 1000U)

Cat. No.: LAMEX-5k (for 5KU)

Cat. No.: LAMEX-25k (for 25KU)

The lyophilized Lambda Exonuclease is also available by inquiry, which can be transported at room temperature.

Description

Lambda Exonuclease acts on double-stranded DNA and gradually cuts off 5´ single nucleotide along the direction of 5´ → 3´. The optimal substrate is 5´ phosphorylated double-stranded DNA, which can also slowly degrade single-stranded DNA and non-phosphorylated substrate. The enzyme cannot be digested from the cut or gap of DNA.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the production of 10 nmol acid-soluble deoxyribonucleotides from double-stranded DNA substrate within 30 minutes in a 50 μl reaction system at 37°C.

Components

Lambda Exonuclease (5 U/μl):    200 μl/1 ml
10×Lambda Exo Buffer:    1 ml/1 ml×5

Storage

The minimum shelf life is 3 years at -20°C.

Note

• 1×Lambda Exo Buffer: 67 mM Glycine-KOH (pH 9.4 @ 25°C), 2.5 mM MgCl₂, 50μg/ml BSA, 37°C incubation.

• The optimal reaction temperature of the enzyme is 37°C, and it can be inactivated at 75°C for 10 minutes.

• The cleavage speed at the 5´-OH end is 20 times slower than that at the 5´-PO₄ end and the cleavage speed of Single-stranded DNA is 100 times slower than that of double-stranded DNA.