All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: HST-500 (for 500U)

Cat. No.: HST-10k (for 10000U)

For ligand-based hot-start DNA polymerase, please visit HM-Taq DNA Polymerase.

We also offer DNA-free HS-Taq DNA Polymerase, which has undergone a rigorous E. coli DNA removal process.

Description

HS-Taq DNA Polymerase is a heat-activated Taq DNA Polymerase modified with monoclonal antibodies. The antibody effectively blocks DNA polymerase activity below 55°C, and irreversible dissociation occurs at high temperatures, restoring polymerase activity, thus effectively avoiding nonspecific amplification at low temperatures. This product is mainly used for fluorescence quantitative PCR, especially Taqman probe method.

Features

• Ideal for routine PCR applications
• Higher specificity and sensitivity
• Avoiding nonspecific amplification and enabling room-temperature reaction setup

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Quality Control

• Nuclease Activity Assay: 5 units (U) of HS-Taq DNA Polymerase were co-incubated with 200 ng of supercoiled plasmid DNA at 37°C for 4 hours. Following incubation, gel electrophoresis was conducted, revealing that less than 10% of the plasmid DNA had been converted to nicked or linear states.
• Non-specific Nuclease Activity Assay: 5 units (U) of HS-Taq DNA Polymerase were incubated with 15 ng of double-stranded DNA fragments at 37°C for 16 hours. Gel electrophoresis was performed to assess the substrate, showing no change in the double-stranded DNA.
• Host DNA Residual Detection: Using Escherichia coli 16S rDNA specific primer probe sets, fluorescence quantitative PCR was employed to detect residual host genomic DNA with 5 units of HS-Taq DNA Polymerase. The Escherichia coli host genome DNA residue was found to be less than 1 copy.

Storage

Instruction: Protocol

Related: HM-Taq DNA Polymerase, Taq Monoclonal Antibody

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory