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Cat. No.: HSDL-2k (for 2KU)

Cat. No.: HSDL-10k (for 10KU)

Description

In addition to possessing the characteristics of DNA ligases, the High-Salt DNA Ligase also has the property of tolerating high salt concentrations. It catalyzes the formation of phosphodiester bonds between the 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA, using NAD+ as a coenzyme as the energy source for the reaction. Ligation only occurs when the oligonucleotide is perfectly complementary to the target DNA, and there are no gaps between them. Compared to other DNA ligases, this enzyme exhibits higher activity at high salt concentrations (equipped with a 2× buffer to increase salt concentration and enhance activity).

Activity Definition

One unit of activity is defined as the amount of enzyme required to ligate 50% of 1 μg of λ DNA fragments (12 bp sticky ends, equivalent to 0.0338 pmole) digested with BstEII within 15 minutes in a 20 μl reaction system at 37°C.

Activity Assay Conditions

20 mM Tris-HCl (pH 7.6), 2.4 M KCl, 25 mM Potassium Acetate, 10 mM Magnesium Acetate, 1 mM NAD+, 10 mM DTT, 0.1% Triton X-100, incubation at 45°C.

Concentration

40 U/μl

Features

• Excellent heat resistance.
• Tolerant to high concentrations of KCl.

Applications

• DNA ligation reactions.
• SNP detection.
• Ligation of cut sites on DNA strands under extremely high KCl concentrations.

Storage

Store at -20°C.

DNA Ligase Products:

• T4 DNA Ligase
• Rapid T4 DNA Ligase
• T4 DNA Ligase (Fast)
• T7 DNA Ligase
• E. coli DNA Ligase
• PBCV-1 DNA Ligase
• T3 DNA Ligase
• Taq DNA Ligase
• Pfu DNA Ligase
• High-Fidelity DNA Ligase
• High-Salt DNA Ligase

SBS Genetech is recognized as one of the global major leading industry players in Ligase Market by third-party market researchers. For more details, please visit Ligase Market - A Global and Regional Analysis Focus on Product, Source, Application, End User, and Country - Analysis and Forecast, 2022-2032