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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: HLUDG2-500 (for 500U)

Cat. No.: HLUDG2-2500 (for 2500U)

The lyophilized Heat-Labile Uracil DNA Glycosylase 2.0 (HL-UDG 2.0) is also available by inquiry, which can be transported at room temperature.

Description

UDG enzyme catalyzes the hydrolysis of the N-glycosidic bond between uracil bases and the ribose-phosphate backbone in DNA chains containing uracil, releasing free uracil. This Uracil DNA Glycosylase (UDG) is derived from the udg gene of a marine cold-adapted bacterium and is obtained through recombinant expression and multi-step protein purification. The enzyme has a molecular weight of 25 kDa and selectively breaks the glycosidic bonds of U bases in single-stranded and double-stranded DNA, with no activity against RNA.

UDG enzyme is commonly used to prevent cross-contamination of PCR products. Its principle of action is based on substituting dTTP with dUTP in the PCR reaction, resulting in PCR products containing dU bases. UDG selectively cleaves the glycosidic bonds of U bases in single-stranded and double-stranded DNA, degrading the PCR products, thereby eliminating contamination issues from previous amplification products in new samples.

Heat-Labile Uracil DNA Glycosylase 2.0 (HL-UDG 2.0) is sourced from a psychrophilic bacterium and exhibits high activity between 15-30°C, becoming inactive at 37-40°C. It is more thermosensitive than our previous HL-UDG, making it ideal for preventing cross-contamination of PCR products. E. coli UDG, on the other hand, becomes partially active after high-temperature inactivation, which is not recommended for preventing cross-contamination of PCR products.

Our HL-UDG 2.0 is recombinantly expressed and purified through multiple steps.

Activity Definition

One unit (U) of activity is defined as the amount of enzyme required to release 60 pmol of uracil bases from uracil-containing double-stranded DNA (dU/dA) per minute at 20°C.

Activity Assay Conditions

Taq PCR Buffer: 10 mM Tris-HCl (pH 8.3, 25°C), 20 mM KCl, 10 mM (NH4)2SO4, 0.01% Tween 20, 1.5 mM MgCl2, incubated at 20°C.

Features

• Exhibits high activity between 10-30°C.
• Becomes inactive starting at 35°C, with 90% inactivation after 10 minutes at 40°C. Complete irreversible inactivation occurs after 15 minutes at 40°C.
• Ideal for preventing cross-contamination of PCR products.
• Uses the same reaction buffer as Taq DNA polymerase, ensuring consistency in the reaction system.

Applications

Excellent enzyme for preventing cross-contamination of PCR products.

Storage

Store at -20°C.

Related:

• Uracil N-Glycosylase
• Heat-Labile Uracil DNA Glycosylase (HL-UDG)
• Heat-Labile Uracil DNA Glycosylase 2.0 (HL-UDG 2.0)
• Thermostable UDG (Uracil-DNA Glycosylase)

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory