GenoJump™ Tn5 Transposase
$465.00 - $1,915.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: GTN5-800 (for 800pmol)
Cat. No.: GTN5-4000 (for 4000pmol)
Description
GenoJump™ Tn5 Transposase is a highly active mutant variant of Tn5 transposase derived from E. coli. It efficiently inserts Tn5 transposons into target sequences, exhibiting high transposition efficiency in both eukaryotic and prokaryotic DNA. This product specifically recognizes DNA fragments with mosaic end sequences (ME), including primers containing ME sequences, to form Tn5 transposomes. The transposomes randomly bind to target DNA and cleave, facilitating the insertion of the carried DNA fragments. Tn5 transposase is widely used in various applications such as in vitro transgenesis (integration of exogenous genes into host cells) and next-generation sequencing (NGS) library preparation.
Transposons, also known as transposable elements (TEs) or jumping genes, are genetic elements capable of "jumping" to different positions within a genome. Although transposons are often referred to as jumping genes, they tend to remain relatively stable at their integration sites, and most transposons eventually lose their jumping activity. Transposons were first discovered by Barbara McClintock in maize and she was awarded the Nobel Prize in Physiology or Medicine in 1983 for her findings. Subsequent research revealed that transposon systems are widespread across the biological kingdom and have been developed as effective tools for genetic analysis.
Compared to the wild-type Tn5 transposase, GenoJump™ Tn5 Transposase exhibits a minimum 1000-fold increase in insertion efficiency. It possesses superior transposition randomness, high stability, and facilitates easy sequencing of the insertion sites.
Application
This product can be used for fragmentation and adapter ligation during library construction for Next-Generation Sequencing (NGS). It facilitates the introduction of sequencing primers into cloned DNA or plasmids, enables the establishment of bacterial gene knockout libraries, facilitates engineering modifications in novel bacterial strains, enables the inactivation of target genes, and allows the insertion of T7 transcription promoters, resistance markers, and other sequences into target DNA.
Enzyme Storage Buffer
50mM HEPES (pH 7.2), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% (v/v) Glycerol.
Reaction Buffer (5X)
50mM HEPES (pH 7.2), 500mM NaCl, 50mM MgCl2.
Stop Buffer (5X)
50mM EDTA (pH 8.0).
For in vitro transposition reactions, using 1μl of GenoJump™ Tn5 Transposase (40μM) per reaction, the small packaging allows for 20 reactions, while the medium packaging allows for 100 reactions.
Precautions
- GenoJump™ Tn5 Transposase (40μM) contains 50% glycerol and will not freeze at -20°C. Avoid storage at -80°C, as it may cause freezing, and repeated freezing and thawing may affect the activity of the Tn5 Transposase.
- GenoJump™ Tn5 Transposase (40μM) has a viscous consistency. When pipetting, ensure accurate sample volume and thoroughly mix after addition to avoid generating bubbles.
- GenoJump™ Tn5 Transposase catalyzes reactions with DNA substrates and is not suitable for RNA experiments.
- This product is intended for bacterial transposition and is not applicable to eukaryotic cells due to the barrier of the nuclear membrane, which hinders transposon insertion. Please design and adjust specific reaction conditions based on relevant literature.
- When conducting bacterial-related research, do not use chemically competent cells, as the success rate of chemical transformation is very low. It is recommended to use electrocompetent cells with an efficiency of no less than 106 cfu/µg.
- This product is for scientific research purposes only and should not be used for clinical diagnosis or treatment, or for food or drug purposes. Do not store it in residential areas.
- For your safety and health, please wear appropriate laboratory attire and disposable gloves when handling this product.
Storage
The minimum shelf life is 1 year at -20°C.
Related:
Only for research and not intended for treatment of humans or animals
