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Cat. No.: F5FSCS-100 (for 100T) 

Description

Fast 5-min 1st Strand cDNA Synthesis Kit (100T) is based on MLV-S, utilizing the stable and efficient 5×Fast RT MasterMix for the reverse transcription of RNA. The reaction process is greatly simplified, requiring only the addition of the template, primers, and RNase-Free H2O, which enhances efficiency and reduces human error during operation. The optimized reverse transcription buffer system allows for higher yields of cDNA, thereby increasing the sensitivity of subsequent detection. The synthesized first-strand cDNA is versatile and can be widely used in second-strand synthesis, hybridization, PCR amplification, and Real-Time PCR. 

The next-generation MLV-S reverse transcriptase is a polymerase lacking RNase H activity and features a unique rapid synthesis domain, which increases the reverse transcription speed of MLV by 3-4 times. Additionally, MLV-S is thermostable, capable of withstanding high-temperature reactions at 50°C, and retains over 30% activity even at 60°C. This feature helps to unwind RNA secondary structures, improving the amplification performance of complex RNA templates.

Components

Note:

1. This product is effective for the reverse transcription of mRNA, tRNA, LncRNA, and ncRNA.
2. This product is not suitable for the reverse transcription of microRNA.

Features

• Utilizes the novel MLV-S, enabling the entire reverse transcription process to be completed in just 6 minutes.
• MLV reverse transcriptase operates at 50°C, providing better transcription of RNA templates with complex secondary structures.
• Highly efficient and convenient reverse transcription premix system.
• The reverse transcription primers are a premix of Random Primer and Oligo dT Primer (RT Primer Mix), ensuring optimal synthesis of various cDNAs from the sample.

Storage

Store at -20°C for up to 2 years; avoid repeated freeze-thaw cycles.

Related: 

• Viral cDNA 1st Strand Synthesis Kit
• Fast 5-min 1st Strand cDNA Synthesis Kit
• PrimeRT First Strand cDNA Synthesis Premix
• PrimeRT Second Strand cDNA Synthesis Kit
• PrimeRT First Strand cDNA Synthesis Premix (with gDNA Clean)

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory