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Cat. No.: EXN3-10k (for 10KU)

Cat. No.: EXN3GF-10k (for 10KU, glycerol free)

The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.

The lyophilized Exonuclease III is also available by inquiry, which can be transported at room temperature.

Description

Exonuclease III was derived from E.coli, with 3 '- 5' exonuclease activity, acts on double-stranded DNA, and gradually cuts single nucleotides from the 3 'OH terminal direction. Each time the enzyme binds to the substrate for catalysis, only a few nucleotides are removed, resulting in progressive deletion within the DNA molecule group. 

The best substrate of this enzyme is the flat end or 5' protruding end double-stranded DNA molecule, but it can also act on the notch of double-stranded DNA, degrading DNA molecules from 3', and releasing 5' single nucleotides. For the 3' protruding end, especially those more than 4nt, it almost does not cut at all, nor can it cut the thiophosphoryl lipid bond. 

The activity of Exonuclease III depends partly on the helical structure and varies according to the sequence (C > A = T > G). In addition, Exonuclease III also has de-purine / pyrimidine endonuclease activity, RNaseH activity, and 3' phosphatase activity.

Applications

• Nondirectional nested deletion

• Preparation of single strand bottom template for dideoxy sequencing

• Preparation of strand-specific probes

• Site-directed mutagenesis

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 50 μl reaction system at 37°C for 30 minutes with 0.15 mM sonicated duplex [3H]-DNA.

Storage

The minimum shelf life is 3 years at -20°C. Avoid repeated freezing and thawing.

Reagents for Recombinase Polymerase Amplification (RPA)

• T4 UvsX Recombinase
• T4 UvsY Protein
• T4 Gene 32 Protein
• Bsu DNA Polymerase
• Sau DNA Polymerase
• Exonuclease III
• Bst DNA/RNA Polymerase
• Endonuclease IV

Instruction: RPA Reaction System (for reference only)