Enhanced Cell Counting Kit-8
$128.00 - $1,268.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: ECCK-5 (for 5ml)
Cat. No.: ECCK-25 (for 25ml)
Cat. No.: ECCK-100 (for 100ml)
Description
Enhanced Cell Counting Kit-8 is a rapid and highly sensitive assay kit widely used for cell proliferation and cytotoxicity based on WST-8. It offers better detection sensitivity and a wider linear range compared to the regular CCK-8 assay kit.
WST-8 is a compound similar to MTT that, in the presence of an electron-coupling reagent, can be reduced by certain dehydrogenases within the mitochondria to produce orange-yellow formazan. The more rapidly cells proliferate, the darker the color becomes, while greater cytotoxicity results in a lighter color. For the same type of cells, the color intensity is linearly related to the number of cells.
WST-8 is an upgraded alternative to MTT and offers distinct advantages when compared to MTT or other MTT-related products such as XTT and MTS. Firstly, the formazan generated by MTT through the reduction by certain dehydrogenases within the mitochondria is not water-soluble and requires a specific dissolution solution; whereas the formazan produced by WST-8, XTT, and MTS is water-soluble, eliminating the need for additional dissolution steps. Secondly, the formazan generated by WST-8 is more readily soluble than that generated by XTT and MTS. Thirdly, WST-8 exhibits greater stability compared to XTT and MTS, resulting in more stable experimental results. Additionally, WST-8 has a wider linear range and higher sensitivity when compared to MTT, XTT, and similar compounds.
In comparison to WST-1, WST-8 offers higher detection sensitivity, greater solubility, and enhanced stability.
Features
- This assay kit can be used for cell proliferation assays induced by cytokines and for cytotoxicity assays induced by anticancer drugs or other cytotoxic agents. It can also be employed for the detection of drug-induced cell growth inhibition.
- The assay using this kit is very convenient. The kit contains a pre-prepared enhanced CCK-8 solution with WST-8, all in a single tube, eliminating the need for any additional preparation steps. There is no requirement for the use of isotopes, and all the assay steps can be completed within the same 96-well plate. Cell washing, cell collection, or additional steps to dissolve formazan are not necessary. It can be used for high-throughput screening of large sample batches.
- Phenol red and serum have no significant impact on the assay results of this kit.
- WST-8 exhibits minimal toxicity to cells. After adding the enhanced CCK-8 solution and developing color, readings can be taken repeatedly at different time intervals using a plate reader, offering flexibility in determining the optimal assay time.
Additional Notes
- Since the assay is conducted using a 96-well plate, it's crucial to be mindful of evaporation issues, especially when the cell culture duration is prolonged. On one hand, as the edges of the 96-well plate are most susceptible to evaporation, you can consider excluding the outermost wells and replace them with an equal volume of PBS, water, or culture medium. On the other hand, you can place the 96-well plate near a water source within the incubator to mitigate evaporation.
- This assay kit relies on a reaction catalyzed by dehydrogenases, so reducing agents (such as certain antioxidants) can interfere with the assay. If there are significant levels of reducing agents in the test system, they should be removed.
- Before using a plate reader for the assay, ensure that there are no air bubbles in each well, as they can interfere with the measurement.
- This product is intended for scientific research use by trained professionals only and should not be used for clinical diagnosis or treatment, nor for food or drug purposes. It should not be stored in residential areas.
- For your safety and health, please wear appropriate laboratory attire and disposable gloves when handling this product.
Storage
The CCK-8 assay kit can be stored at 4°C, with a shelf life of 1 year. Alternatively, it can be stored at -20°C, with a shelf life of 2 years. Please store the kit in a light-protected manner.
Related:
- Cell Counting Kit-8 (CCK-8)
- Enhanced Cell Counting Kit-8
- Super-Enhanced Cell Counting Kit-8
- Cell Counting Kit-3D
Only for research and not intended for treatment of humans or animals
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