All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: ECLP-100 (for 100ml)

Cat. No.: ECLP-500 (for 500ml)

Description

ECL Plus is an ultra-sensitive ECL chemiluminescent reagent kit. This product reacts chemically with horseradish peroxidase conjugated to the secondary antibody, emitting fluorescence. This fluorescence can then be detected using X-ray film or other suitable chemiluminescence imaging equipment.

We offer four types of Western chemiluminescent detection reagents: ECL Plus, ECL Star, ECL Moon, and ECL SuperMoon. For routine Western detection, we recommend using ECL Star. For detecting proteins with relatively high abundance, such as internal reference proteins, the more cost-effective ECL Plus can also be used. For low-abundance and difficult-to-detect target proteins, we recommend using the highly sensitive ECL Moon or the even more sensitive ECL SuperMoon. However, for detecting proteins with moderate abundance, we do not recommend using ECL Moon or ECL SuperMoon, as they are prone to causing overexposure.

Performance

ECL Plus has high sensitivity, being at least 200 times more sensitive than DAB staining, about 20-50 times more sensitive than Amersham’s ECL or previously produced ECL, and 3-100 times more sensitive than Pierce’s SuperSignal West Pico Substrate.

ECL Plus also has a longer luminescence duration. When 100 µL of 1 µg/mL horseradish peroxidase-labeled IgG is tested with 100 µL of ECL Plus working solution or Pierce’s SuperSignal West Pico Substrate working solution, both emit visible blue fluorescence in a darkroom. However, the brightness of the tube with ECL Plus working solution is significantly higher. During the first few minutes, the luminescence intensity of the tube with ECL Plus working solution is three times that of the tube with SuperSignal West Pico Substrate working solution. After about 30 minutes, the tube with ECL Plus working solution still emits strong visible blue fluorescence, while the fluorescence from the tube with SuperSignal West Pico Substrate working solution becomes very difficult to discern with the naked eye. Quantitative results show that at this point, the luminescence intensity of the tube with ECL Plus working solution is over 100 times that of the tube with SuperSignal West Pico Substrate working solution.

ECL Plus's high sensitivity significantly conserves precious samples as well as expensive primary and secondary antibodies. It is important to appropriately reduce the amount of primary and secondary antibodies or samples to avoid producing overly intense bands that are difficult to compare.

ECL Plus features a new formulation that is more stable. When incubated in a 37°C water bath for 30 days, the two reagents of ECL Plus show no significant difference in detection results compared to the same reagents stored at 4°C.

ECL Plus has a very low background and is suitable for both PVDF membranes and nitrocellulose membranes. PVDF membranes and nitrocellulose membranes can be ordered from us.

Components

• ECL Plus Solutions A
• ECL Plus Solutions B

Storage Conditions

Store at 4°C in the dark for one year. If stored at 37°C in the dark, it is effective for one month. For long-term storage, keep at -20°C in the dark. At -20°C, it can be preserved for a longer period.

Notes

• When handling ECL Plus Solutions A and B, always change the pipette tip to avoid cross-contamination, which can cause Solutions A or B to become ineffective over time and affect subsequent performance.
• After use, tightly close the bottle caps to prevent degradation. This is especially important for Solution B, which contains oxidizers and is more prone to reduction and degradation.
• ECL Plus has a long fluorescence duration, but it is strongest within the first 30 minutes after the reaction starts. The fluorescence will gradually diminish after this period, so make sure to take full advantage of this stronger fluorescence window for imaging.
• If the sensitivity of ECL Plus is not sufficient for your experiment, consider using the more sensitive ECL Star or ECL Moon.
• Both ECL Plus Solutions A and B are harmful to humans. Handle with care and use appropriate protection to avoid direct contact with skin or inhalation.
• This product is for scientific research use by professionals only and is not intended for clinical diagnosis or treatment, food or drug use, and should not be stored in residential areas.
• For your safety and health, please wear a lab coat and disposable gloves while handling the product.