All products have special prices for bulk purchase, please contact for more details if required.

Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: ECDL-2k (for 2KU)

Cat. No.: ECDL-10k (for 10KU)

Description

Ec DNA Ligase is derived from Escherichia coli (E. coli) with a molecular weight of 77 kDa. It is a DNA ligase enzyme that catalyzes the formation of phosphodiester bonds between the 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA. It functions similarly to T4 DNA Ligase but uses NAD+ as a coenzyme. The optimal reaction pH is 7.5-8.0 (in Tris-HCl buffer). Unlike T4 DNA Ligase, this enzyme can only catalyze the ligation of sticky-ended DNA, but it can also ligate blunt-ended DNA under conditions of PEG and high concentrations of monovalent cations. Unlike T4 DNA Ligase, which can ligate 5'-P ends of DNA to 3'-OH ends of RNA and 5'-P ends of RNA to 3'-OH ends of DNA, this enzyme cannot catalyze the ligation between DNA and RNA or between two RNA molecules. It is not active on single-stranded DNA or RNA or on blunt-ended DNA. Due to some degree of ligation even when the oligonucleotide is incompletely paired with the target DNA, it is not recommended for precise SNP detection. Our Ec DNA Ligase is recombinantly expressed and prepared through multi-step purification of the recombinant protein.

Activity Definition

One unit is defined as the amount of enzyme required to ligate 50% of 6 µg of λ DNA fragments (with a 5' end concentration of 0.12 μM or 300 μg/ml) digested with HindIII within 30 minutes under the reaction conditions of a 20 μl 1× Ec DNA Ligase Buffer at 16°C.

Activity Assay Conditions

30 mM Tris-HCl (pH 8.0 @ 25°C), 4 mM MgCl2, 1 mM DTT, 26 µM NAD+, 50 µg/mL BSA, reaction temperature at 16°C.

Features

• Preferential ligation of gaps in dsDNA rather than significant ligation of two dsDNA fragments.
• Heat inactivation: Heating at 65°C for 20 minutes.

Applications

• Repair of dsDNA nicks.
• Okayama and Berg cDNA cloning.

Storage

Store at -20°C.

DNA Ligase Products:

• T4 DNA Ligase
• Rapid T4 DNA Ligase
• T4 DNA Ligase (Fast)
• T7 DNA Ligase
• E. coli DNA Ligase
• PBCV-1 DNA Ligase
• T3 DNA Ligase
• Taq DNA Ligase
• Pfu DNA Ligase
• High-Fidelity DNA Ligase
• High-Salt DNA Ligase

SBS Genetech is recognized as one of the global major leading industry players in Ligase Market by third-party market researchers. For more details, please visit Ligase Market - A Global and Regional Analysis Focus on Product, Source, Application, End User, and Country - Analysis and Forecast, 2022-2032