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Cat. No.: FDMM-3k (for 3KU) 

Cat. No.: FDMM-15K (for 15KU) 

Description

DPolUltra Thermo-stable M-MLV Reverse Transcriptase is an RNA-guided DNA polymerase completely designed by bioinformatics, and expressed by recombinant E.coli strain. This enzyme has below characteristics:

• Strong Reverse Transcriptase Activity: DPolUltra Thermo-stable M-MLV Reverse Transcriptase can use single-stranded RNA to synthesize cDNA. Thanks to the continuous optimization by enzyme engineering, its reverse transcriptional amplification capability for rare bases has been greatly enhanced, greatly increasing the proportion of full-length cDNA molecules and the average length.
• DNA Polymerase Activity: DPolUltra Thermo-stable M-MLV Reverse Transcriptase uses single-stranded DNA as a template to synthesize complementary DNA strands.
• Warm Start Property & Thermal Stability: The activity below 37°C is lower than 5% of the optimal temperature activity. With the temperature gradually increasing, it starts to show strong reverse transcriptase activity and DNA polymerase activity and can maintain this activity at 65°C for more than 30 minutes. The reaction system can be prepared at room temperature, which greatly improves the specificity of the amplification reaction and the consistency within the batch during the high-throughput reaction.
• Complete RNase H activity. 

The above characteristics make DPolUltra Thermo-stable M-MLV Reverse Transcriptase perfectly applicable to the detection of RNA in the amplification reaction, especially in RT-qPCR and RT-LAMP experiments, which can effectively improve the sensitivity, specificity, and product yield of amplification.

Components

• DPolUltra Thermo-stable M-MLV Reverse Transcriptas (15 U/μl): 200 μL
• 10 × Buffer: 1 mL
• MgSO₄(100mM): 1 ml

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of dTTP into acid-insoluble substances within 20 minutes at 50°C.

Applications

• cDNA synthesis
• RT-LAMP
• RT-qPCR

Storage

The minimum shelf life is 1 year at -20°C.

Notes

• Storage Buffer: 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, pH 7.5 @ 25°C.
• 10 × Buffer: 200 mM Tris-HCl, 500 mM KCl, 100 mM (NH₄)₂SO₄, 20 mM MgSO₄,  1% Tween-20, pH 8.8 @ 25°C.

Related: Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-), TGI-RTase Reverse Transcriptase, Thermo-stable M-MLV Reverse Transcriptase (RNase H-)

Only for research and not intended for treatment of humans or animals

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