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Cat. No.: EUN-1 (for 1ml)

Cat. No.: EUN-5 (for 5ml)

Description

dNTP/dUTP Mix (2.5mM each/5mM) is a mixed aqueous solution of dATP, dCTP, dGTP, and dUTP. The final concentrations of dATP, dCTP, and dGTP are 2.5 mM each, while the final concentration of dUTP is 5 mM. This product is commonly used in PCR, real-time PCR, RT-PCR involving enzymes such as Taq DNA polymerase, as well as primer extension, cDNA synthesis, and other applications. One of its common uses is in conjunction with Uracil DNA Glycosylase (UDG enzyme), which recognizes and cleaves sites containing dUTP, to prevent false positives in PCR amplification due to contamination of PCR products.

This product is suitable for PCR amplification involving members of the A family of polymerases, such as Taq polymerase, and can also be used in isothermal amplification involving Bst DNA Polymerase. Enzymes that can utilize dATP, dTTP, dGTP, and dCTP may not necessarily be compatible with dUTP; specific enzyme compatibility should be referenced in the enzyme's product documentation or determined through testing.

The dNTP/dUTP Mixture is often used in combination with Uracil DNA Glycosylase (UDG enzyme) to establish a dUTP/UDG enzyme contamination prevention system. The UDG enzyme removes previous amplification products containing deoxyuracil (dU), which may contaminate subsequent gene amplification detection systems, effectively reducing false positives during PCR, qPCR, and isothermal amplification reactions.

This dNTP/dUTP Mixture is prepared with ultrapure water and pH-adjusted to 7.0 using a high-purity NaOH solution.

Features

The product is free from DNase, RNase, phosphatases, and proteases, making it suitable for various routine molecular biology experiments including gene detection.

Storage

Store at -20°C.

Precaution

• For environmental contaminants containing gene amplification products that do not contain dU, Uracil DNA Glycosylase (UDG enzyme) cannot exert its effect. In such cases, considering redesigning primers to target different amplification regions and establishing a dUTP/UDG enzyme contamination prevention system can effectively reduce false positives caused by environmental contamination from gene amplification products.
• It can be dissolved at room temperature. After dissolution, it is advisable to store it in a cooler or on an ice bath. After use, it should be promptly stored at -20°C.
• This product is intended for scientific research by professionals only and is not intended for clinical diagnosis or treatment. It must not be used for food or drugs and must not be stored in residential settings.
• For your safety and health, please wear laboratory attire and disposable gloves when handling.

Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.

Only for research and not intended for treatment of humans or animals

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