All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DNIST-1k (for 1KU)

Cat. No.: DNIST-25k (for 25KU)

Description

This product is a recombinant expression-derived DNase I. Compared to the wild type, this product has undergone protein engineering modifications, resulting in significantly improved salt tolerance, being able to completely digest DNA in a solution of 300 mM monovalent salt. This product is free from RNase contamination.

Definition of Activity

1 unit (U) of activity is defined as the amount of enzyme required to completely degrade 1 μg of pUC19 plasmid DNA at 37°C for 10 minutes.

Application

• In vitro transcription: Used for removal of template DNA after in vitro transcription using RNA polymerase.
• Removal of genomic DNA contamination from RNA samples.
• Used in DNase I footprinting analysis of DNA-protein interactions.
• Used in conjunction with DNA Polymerase I for nick translation labeling of DNA.
• Used in construction of DNA random fragment libraries.
• Partial cleavage of genomic DNA in cell apoptosis TUNEL detection as a positive control.

Quality Control

• Protein Purity: Protein purity is assessed using SDS-PAGE gel electrophoresis, with a minimum purity of 90%.
• RNase Residual Detection: 2 units of DNase I-ST, RNase-free are incubated with 500 ng total RNA at 37°C for 1 hour. Gel electrophoresis is then conducted, ensuring that over 90% of the RNA remains intact.

Storage

-20°C.

Comparison

Related:

• DNase I, RNase-free (from Bovine pancreas)
• DNase I, RNase-free (GMP) (from yeast)
• DNase I, RNase-free (Lyophilized) (from yeast)
• DNase I-ST, RNase-free (from yeast)

SBS Genetech is recognized as one of the global major leading industry players in mRNA Raw Material by third-party market researchers. For more details, please visit Top 5 mRNA Vaccine & Therapeutics Raw Material Market Companies in Global Market 2022.

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory