![DNase I-ST, RNase-free](https://user-images.strikinglycdn.com/res/hrscywv4p/image/upload/c_limit,fl_lossy,h_1000,w_500,f_auto,q_auto/2311560/327637_508188.png)
DNase I-ST, RNase-free
$100.00 - $300.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: DNIST-1k (for 1KU)
Cat. No.: DNIST-5k (for 5KU)
Description
This product is a recombinant expression-derived DNase I. Compared to the wild type, this product has undergone protein engineering modifications, resulting in significantly improved salt tolerance, being able to completely digest DNA in a solution of 300 mM monovalent salt. This product is free from RNase contamination.
Definition of Activity
1 unit (U) of activity is defined as the amount of enzyme required to completely degrade 1 μg of pUC19 plasmid DNA at 37°C for 10 minutes.
Application
- In vitro transcription: Used for removal of template DNA after in vitro transcription using RNA polymerase.
- Removal of genomic DNA contamination from RNA samples.
- Used in DNase I footprinting analysis of DNA-protein interactions.
- Used in conjunction with DNA Polymerase I for nick translation labeling of DNA.
- Used in construction of DNA random fragment libraries.
- Partial cleavage of genomic DNA in cell apoptosis TUNEL detection as a positive control.
Quality Control
- Protein Purity: Protein purity is assessed using SDS-PAGE gel electrophoresis, with a minimum purity of 90%.
- RNase Residual Detection: 2 units of DNase I-ST, RNase-free are incubated with 500 ng total RNA at 37°C for 1 hour. Gel electrophoresis is then conducted, ensuring that over 90% of the RNA remains intact.
Storage
-20°C.
Related:
Only for research and not intended for treatment of humans or animals
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