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Cat. No.: DNIST-1k (for 1KU)

Cat. No.: DNIST-5k (for 5KU)

Description

This product is a recombinant expression-derived DNase I. Compared to the wild type, this product has undergone protein engineering modifications, resulting in significantly improved salt tolerance, being able to completely digest DNA in a solution of 300 mM monovalent salt. This product is free from RNase contamination.

Definition of Activity

1 unit (U) of activity is defined as the amount of enzyme required to completely degrade 1 μg of pUC19 plasmid DNA at 37°C for 10 minutes.

Application

• In vitro transcription: Used for removal of template DNA after in vitro transcription using RNA polymerase.
• Removal of genomic DNA contamination from RNA samples.
• Used in DNase I footprinting analysis of DNA-protein interactions.
• Used in conjunction with DNA Polymerase I for nick translation labeling of DNA.
• Used in construction of DNA random fragment libraries.
• Partial cleavage of genomic DNA in cell apoptosis TUNEL detection as a positive control.

Quality Control

• Protein Purity: Protein purity is assessed using SDS-PAGE gel electrophoresis, with a minimum purity of 90%.
• RNase Residual Detection: 2 units of DNase I-ST, RNase-free are incubated with 500 ng total RNA at 37°C for 1 hour. Gel electrophoresis is then conducted, ensuring that over 90% of the RNA remains intact.

Storage

-20°C.

Comparison

Related:

• DNase I, RNase-free (from Bovine pancreas)
• DNase I, RNase-free (GMP) (from yeast)
• DNase I, RNase-free (Lyophilized) (from yeast)
• DNase I-ST, RNase-free (from yeast)

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Only for research and not intended for treatment of humans or animals

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