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Cat. No.: DNIGMP-10k (for 10KU)

Cat. No.: DNIGMP-100k (for 100KU)

Description

DNase I, RNase-free (GMP), is an endodeoxyribonuclease that catalyzes the degradation of both single- and double-stranded DNA randomly, yielding 5'-phosphate terminal oligonucleotides. Its activity is contingent upon Ca2+ but can be further potentiated by bivalent metal ions such as Mg2+ and Mn2+. In the presence of Mg2+, the enzyme exhibits the capability to randomly cleave any site on either strand of double-stranded DNA. Conversely, in the presence of Mn2+, DNase I demonstrates a higher affinity for nearly identical sites on both strands of DNA, resulting in the generation of DNA fragments with either blunt ends or sticky ends, characterized by 1 to 2 nucleotide overhangs.

DNase I, RNase-free (GMP) is the GMP-compliant version.

Definition of Activity

One active unit is defined as the amount of enzyme which will completely degrade 1 µg of pUC19 DNA in 10 minutes at 37℃ in DNase I Reaction  Buffer.

Application

• Elimination of contaminating genomic DNA from RNA samples.
• Degradation of DNA templates in transcription reactions.
• DNase I footprinting analysis.
• Nick translation for DNA labeling.
• Construction of DNA libraries for diverse applications.

Quality Control

• Protein Purity: The enzyme is ≥95% pure as determined by SDS-PAGE analysis using Coomassie Blue staining.
• RNase Activity: A 10 µl reaction containing 500 ng of total RNA and 1 U of DNase I, RNase-free incubated for 1 hours at 37℃ results in >90% of the substrate RNA remains intact as determined by agarose gel electrophoresis.

Storage

-20°C.

Comparison

Related:

• DNase I, RNase-free (from Bovine pancreas)
• DNase I, RNase-free (GMP) (from yeast)
• DNase I, RNase-free (Lyophilized) (from yeast)
• DNase I-ST, RNase-free (from yeast)

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