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Cat. No.: DGEMB-50 (for 50T)

Cat. No.: DGEMB-200 (for 200T)

Description

DNA Gel Extraction Kit with Magnetic Beads is a reagent kit that utilizes a novel nucleic acid purification medium coated with magnetic beads, designed for the stable, efficient, and convenient recovery of target DNA from agarose gel.

This kit is suitable for the recovery of DNA fragments from agarose gels after gel electrophoresis, including PCR products, plasmid DNA fragments cut by enzymes, linearized products of supercoiled plasmid DNA after enzyme digestion, and DNA ligation products. The purified DNA obtained can be directly used for subsequent processes such as restriction digestion, ligation, bacterial transformation, sequencing, PCR, hybridization, and more.

The agarose gel rapidly dissolves in the melting solution, allowing the DNA to be fully released. Subsequently, the DNA specifically binds to the magnetic beads. Under the influence of an external magnetic field (such as a magnetic separation rack), the magnetic beads can be rapidly and efficiently separated from the corresponding solution. After thorough washing to remove impurities, the DNA is finally eluted from the magnetic beads using elution buffer, resulting in highly purified target DNA samples.

Components

• Magnetic Beads
• Solution I (Melting Buffer)
• Solution II (Wash Buffer) 
• Solution III (Elution Buffer)

Features

• This kit offers several advantages, including rapid gel melting, flexible and convenient operation, high recovery efficiency, good purity, and the ability to recover a wide range of DNA fragment sizes. Depending on the specific conditions, the amount of magnetic beads can be flexibly adjusted, typically allowing the recovery of target DNA fragments to be completed within 20 minutes. It is suitable for purifying DNA fragments larger than 100 base pairs, and it can also achieve satisfactory results for purifying DNA fragments as large as 10 kilobases. Typically, the recovery rate ranges from 60% to 80%, with a decrease in recovery rate observed for fragments smaller than 200 base pairs or larger than 10 kilobases.
• Currently, the primary method for DNA gel recovery is column-based recovery. This method typically requires repetitive centrifugation or specialized filtration devices, and the fiber cutting that occurs during column purification is not particularly conducive to the recovery of large DNA fragments. In contrast, the magnetic bead method operates under mild conditions, eliminating the need for cumbersome centrifugation or filtration steps and replacing them with a simple magnetic adsorption process, ensuring a quick and convenient operation.
• Compared to traditional purification methods, this kit does not involve the use of toxic reagents like phenol/chloroform.
• The melting buffer of this kit has a strong buffering capacity and has been tested not to cause significant changes in system pH during gel melting, which could otherwise lead to a decreased binding capacity between DNA and magnetic beads. Therefore, there is no need to add phenol red indicator to correct specific pH conditions in the system.

Storage

Store at room temperature, effective for one year. When Magnetic Beads are not in use for an extended period, they can be stored at 4°C, which allows for even longer storage.

Note

• You will need to provide anhydrous ethanol and a magnetic separation apparatus. 
• Before the first use, add the specified amount of anhydrous ethanol to each bottle of Solution II (Wash Buffer), mix well, and label the bottles accordingly.
• Magnetic bead suspension may settle when left undisturbed. Before use, ensure thorough mixing by vortexing or inverting several times.
• Before magnetic separation, gently shake the centrifuge tube to disperse the magnetic beads fully before bringing them close to the magnetic field. If bead aggregation occurs, gently agitate the liquid inside the tube to allow the adhered beads to flow down.
• This product is suitable for manual extraction and can also be used with workstations or nucleic acid extraction instruments.
• To improve recovery efficiency, it is recommended to change the electrophoresis buffer during electrophoresis and reduce the voltage appropriately to prevent solution heating during electrophoresis. When excising the gel, minimize UV exposure time to reduce DNA damage and remove gel portions that do not contain DNA as much as possible.
• Deionized water can be used instead of the elution buffer, but the pH of the deionized water should not be lower than 6.5. If it is lower, adjust it to 7.5-8.5 with a low-concentration NaOH solution.
• Solution I (Melting Buffer) may have some irritant effects on the human body. Please handle it with care and take appropriate precautions.
• This product is for research use by professionals only and should not be used for clinical diagnosis or treatment. It should not be used in food or drugs and should not be stored in residential homes.
• For your safety and health, wear lab attire and disposable gloves while handling the product.

Only for research and not intended for treatment of humans or animals

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