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Cat. No.: DFRNT-200 (for 1200U)

Cat. No.: DFRNT-1k (for 1000U)

Description

DNA-Free RNase T, also known as Exonuclease T (Exo T), is a nuclease specific for single-stranded RNA or DNA. This enzyme requires a free 3' end and removes nucleotides in the 3' to 5' direction. RNase T exhibits ten times higher activity on DNA than RNA and can be used to blunt the ends of RNA or DNA molecules with 3' overhangs. It is a mesophilic enzyme and can be inactivated by incubation at 65℃ for 20 minutes.

Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to hydrolyze 1 nmol of [3H]-labeled poly(thymidylic acid) to 0.1 nmol of trichloroacetic acid-soluble nucleotides in 30 minutes at 25℃ in a 1× AM Buffer D reaction system.

Features

• Completely free from bacterial DNA.
• Removes 3' overhangs of double-stranded DNA, creating blunt ends.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory