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Cat. No.: DFP29P-1k (for 1000U)

Cat. No.: DFP29P-5k (for 5000U)

Description

DNA-Free phi29 DNA Polymerase is a DNA polymerase encoded by the phi29 bacteriophage of the thermophilic Bacillus subtilis. This enzyme exhibits excellent strand displacement and continuous synthesis properties, along with 3´→5´ exonuclease proofreading activity. Our phi29 DNA polymerase is recombinantly expressed and prepared through multiple purification steps, devoid of genomic and plasmid DNA from the production strain. 

Unit Definition

One unit of activity is defined as the amount of enzyme required to incorporate 0.5 pmol dNTP into acid-insoluble material within 10 minutes at 30℃ in 1× phi29 DNA Polymerase reaction buffer.

Features

• Completely free from bacterial DNA.
• Lacks 5’→3’ exonuclease activity.
• Superior strand displacement capability.
• Exceptional continuous synthesis ability.
• High amplification fidelity.
• Heat inactivation: 65℃ for 10 minutes.

Applications

• Gap filling (without nick translation activity).
• Strand displacement and DNA continuous synthesis reactions.
• High-fidelity rapid rolling circle replication at room temperature.
• High-fidelity synthesis of long single-stranded DNA molecules.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory