All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: FOHRPM-1 (for 1ml)

Cat. No.: FOHRPM-5 (for 5ml)

Cat. No.: FOHRPM-20 (for 20ml)

Cat. No.: FOHRPM-50 (for 50ml)

Description

DNA-Free One-Step High Temperature RT-PCR 2× SuperMix is a specialized premix for RT-PCR reactions. It can be used for the amplification and qualitative analysis of RNA molecules, including total RNA, mRNA, ribosomal RNA, tRNA, and more. This product contains MMLV reverse transcriptase 2.0, RNase Inhibitor, hot-start Taq DNA polymerase, dNTPs, and an optimized reaction buffer at a 2× concentration. One of the core components, MMLV RTase 2.0, has been genetically engineered to significantly improve its thermal stability, making it resistant to 55-60℃. This allows the reverse transcription reaction to be performed at 50-55℃, eliminating the inhibitory effects of RNA secondary structures on reverse transcription. After converting the RNA strand into a cDNA strand with MMLV2.0, the hot-start Taq DNA polymerase uses the cDNA strand as a template to synthesize double-stranded DNA. The buffer is specifically optimized for RT-PCR, making it highly suitable for one-step RT-PCR reactions. For RT-PCR, simply add the RNA template, primers, and water to adjust the 2× SuperMix solution to a 1× concentration for the reaction.

Features

• Completely free from bacterial DNA.
• One-step high-temperature RT-PCR reaction, simple operation, avoiding contamination from opening tubes.
• Contains RNase inhibitors to prevent RNA degradation.
• The reaction buffer is optimized to accommodate both RNase and Taq DNA polymerase reaction conditions.
• The reverse transcription reaction can be performed at 50-55℃, eliminating the inhibitory effects of RNA secondary structures.

Applications

• RT-PCR amplification of target RNA and preparation of double-stranded DNA from mRNA and other RNA molecules.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

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