All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DFKPDP-250 (for 250U)

Cat. No.: DFKPDP-1250 (for 1250U)

Cat. No.: DFKPDP-5k (for 5KU)

Cat. No.: DFKPDP-12500 (for 12500U)

Description

DNA-Free KOD Plus DNA polymerase is a thermostable DNA polymerase derived from thermophilic archaea, recombinantly expressed in Escherichia coli, with a molecular weight of 90 kDa. Its fidelity is approximately 60 times higher than that of ordinary Taq DNA polymerase. This enzyme has undergone multiple special modifications to possess strong amplification ability and extension speed, with an amplification fragment length of 8-15 kb and an extension speed of 3-6 kb/min (at 72℃). It exhibits 5’→3’ polymerase activity and 3’→5’ exonuclease activity. The amplification products have blunt ends and are not suitable for TA cloning.

Our DNA-Free HS KOD DNA polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-free Pfu particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Unit Definition

The amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into acid-insoluble material within 30 minutes at 72℃ using salmon sperm DNA as the template/primer is defined as one unit of activity (U).

Features

• Completely free from bacterial DNA.
• Specially modified through multiple steps to enhance amplification capability and extension speed, effectively amplifying 8-15 kb DNA fragments under 3 kb/min extension conditions.
• Good thermal stability: Half-life exceeds 40 minutes at 98℃.
• Highest fidelity: 60 times the fidelity of Taq DNA polymerase.
• Tolerant to dUTP and dITP.
• PCR products have blunt ends and can be directly used for blunt-end cloning.

Applications

• High-fidelity PCR amplification.
• Preparation of PCR products for gene cloning or protein expression.
• Blunt-end PCR cloning (removing 3’ overhangs).
• Site-specific mutagenesis.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory