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Cat. No.: DFEKMD1-200 (for 200U)

Cat. No.: DFEKMD1-1k (for 1000U)

Description

DNA-Free Klenow fragment (exo-) of EcDNApol I (EcDNApolI KF (exo-)) is a truncated version of the Klenow fragment of E. coli DNA polymerase I. It retains polymerase activity but has lost 5´→3´ exonuclease activity, and site-directed mutations (D355A, E357A) have eliminated its 3´→5´ exonuclease activity.

Our DNA-Free Klenow fragment (exo-) of EcDNApol I is a recombinant protein and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol dNTP into acid-insoluble material within 30 minutes at 37℃ under 1× EcDNApolI KF (exo-) reaction buffer conditions.

Features

• Completely free from bacterial DNA.
• Lacks 3´→5´ exonuclease activity.
• Moderate strand displacement activity.
• Heat inactivation at 75℃ for 20 minutes.

Applications

• Generation of probes using random primers.
• Random primed labeling.
• Sanger dideoxy sequencing.
• Second-strand cDNA synthesis.
• Second-strand synthesis in mutagenesis protocols.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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