All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DFHST-250 (for 250U)

Cat. No.: DFHST-1250 (for 1250U)

Cat. No.: DFHST-5k (for 5KU)

Cat. No.: DFHST-12500 (for 12500U)

For ligand-based hot-start DNA polymerase, please visit HM-Taq DNA Polymerase.

Description

DNA-Free HS-Taq DNA Polymerase is a HotStart Taq DNA Polymerase, activated by heating at 95°C for 5 minutes or 98°C for 2 minutes. Taq DNA Polymerase is a thermostable DNA polymerase derived from the thermophilic bacterium Thermus aquaticus, recombinantly expressed in E. coli, with a molecular weight of 94 kDa. This enzyme has been specially modified to have superior amplification capability and extension speed, capable of amplifying fragments up to 5-6 kb with an extension speed of 2-3 kb/min (72°C). It possesses 5’→3’ polymerase activity and weak 5’→3’ exonuclease activity, but lacks 3’→5’ exonuclease activity. The amplification products have 3’-dA overhangs.

Our DNA-Free HS-Taq DNA Polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-free HS-Taq DNA Polymerase particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Features

• Completely free from bacterial DNA.
• Versatile buffer system suitable for all applications.
• High sensitivity, enabling efficient amplification genes.
• High productivity, ensuring high yields of products.
• Avoiding nonspecific amplification and enabling room-temperature reaction setup

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Storage

Related: HS-Taq DNA Polymerase

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory