All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DFHSFT-250 (for 250U)

Cat. No.: DFHSFT-1250 (for 1250U)

Cat. No.: DFHSFT-5k (for 5KU)

Cat. No.: DFHSFT-12500 (for 12500U)

Description

DNA-Free HS HiFi Taq DNA Polymerase is the hot-start version of HiFi Taq DNA Polymerase, requiring activation by heating at 95°C for 5 minutes or at 98°C for 2 minutes. It enhances amplification specificity and reduces non-specific amplification and primer-dimer formation. HiFi Taq DNA Polymerase is a high-efficiency PCR enzyme developed based on the Barnes method. It combines KOD DNA polymerase, with strong 3'→5' exonuclease activity (proofreading activity), and Taq DNA polymerase in an optimal ratio, achieving superior PCR performance and improved fidelity. Both enzymes are specially modified for enhanced amplification capability and extension speed, with fragment lengths up to 5-6 kb and an extension rate of 1-2 kb/min (72°C). The enzyme exhibits 5'→3' polymerase activity, weak 5'→3' exonuclease activity, and optimized 3'→5' exonuclease activity. A small amount of amplification product has 3'-dA overhangs.

Our DNA-Free HS HiFi Taq DNA Polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes this polymerase particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Features

• Completely free from bacterial DNA.
• Specially modified for enhanced amplification capability, effectively amplifying 3-5kb DNA fragments at a rate of 2kb/min.
• Enhances amplification specificity, reducing non-specific amplification and primer-dimer formation.
• Excellent thermal stability: Half-life exceeds 40 minutes at 95°C.
• A small amount of PCR product has 3'-dA overhangs, making direct T/A cloning slightly less efficient.
• Broad tolerance range for template DNA.

Applications

• Conventional PCR amplification
• Colony PCR
• Multiplex PCR
• DNA fluorescence labeling

Unit Definition

The amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into acid-insoluble material within 30 minutes at 72°C, using salmon sperm DNA as the template/primer, is defined as one activity unit (U).

Storage

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory