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Cat. No.: DFEPA-100 (for 100U)

Cat. No.: DFEPA-500 (for 500U)

Description

DNA-Free E. coli Poly(A) Polymerase catalyzes the addition of ATP to the 3’ end of single-stranded RNA (ssRNA) primers without the need for a template, typically adding 20-200 consecutive AMP residues to form a polyA tail. This polyA tail can enhance mRNA stability. Adding a polyA tail to RNAs that naturally lack one (such as microRNAs) provides an oligo-dT primer binding site for cDNA synthesis.

Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit of activity is defined as the amount of enzyme required to incorporate 1 nmol AMP into 16nt ssRNA within 10 minutes at 37℃ under 1× Poly(A) Polymerase reaction buffer conditions.

Features

• Completely free from bacterial DNA.
• 3’ end labeling of RNA.
• Adding a polyA tail to microRNAs to provide an oligo-dT primer binding site for cDNA synthesis.
• Adding a 3’ polyA tail to in vitro transcribed RNA molecules to enhance RNA stability and improve translation efficiency in cells.
• Labeling RNA with 5’ triphosphate cordycepin (cordycepin 5´-triphosphate) or ATP.
• Adding a polyA tail to RNA for cloning or affinity purification.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory