All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DFBULF-200 (for 200U)

Cat. No.: DFBULF-1k (for 1000U)

Description

DNA-Free Bsu DNA polymerase (Large Fragment) is the large fragment of Bsu DNA polymerase, retaining the 5´→3´ polymerase activity of Bacillus subtilis DNA polymerase A but lacking the 5´→3´ exonuclease domain. This large fragment also lacks 3´→5´ exonuclease activity. Due to the absence of 5´→3´ exonuclease activity, this enzyme exhibits strong strand displacement synthesis activity.

Our DNA-Free Bsu DNA polymerase (Large Fragment) is a recombinant protein and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol dNTP into acid-insoluble material within 30 minutes at 37℃ in 1× Buffer.

Features

• Completely free from bacterial DNA.
• Strong strand displacement synthesis activity.
• No nick translation activity.
• Heat inactivation at 75℃ for 20 minutes.

Applications

• RPA isothermal DNA amplification.
• Strand displacement DNA synthesis.
• Random primed labeling.
• Second-strand cDNA synthesis.
• Addition of a single dA tail.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory