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Cat. No.: DFBFL-500 (for 500U)

Cat. No.: DFBFL-2500 (for 2500U)

Description

DNA-Free Bst DNA polymerase (Full Length) is a full-length DNA polymerase derived from the thermophilic bacterium Bacillus stearothermophilus. It exhibits 5´→3´ DNA polymerase and 5´→3´ exonuclease activities but lacks 3´→5´ exonuclease activity. This enzyme has very strong 5´→3´ exonuclease activity, making it unsuitable for strand displacement but ideal for nick translation reactions on double-stranded DNA, especially at high temperatures. Taq DNA polymerase, on the other hand, has weak 5´→3´ exonuclease activity and is not suitable for nick translation. Additionally, Bst DNA polymerase can be used for primer extension reactions at high temperatures, similar to Taq DNA polymerase.

Our DNA-Free Bst DNA polymerase (Full Length) is a recombinant protein expressed in E. coli and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol dNTP into acid-insoluble material within 30 minutes at 65℃ under 1× Buffer reaction conditions.

Features

• Completely free from bacterial DNA.
• Strong 5´→3´ exonuclease activity, not suitable for strand displacement.
• Lacks 3´→5´ exonuclease activity.
• Heat inactivation at 85℃ for 5 minutes.
• Suitable for high-temperature nick translation reactions for GC-rich DNA amplification.
• Primer extension reactions at high temperatures.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

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