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Cat. No.: DFTAB5-500 (for 500U)

Cat. No.: DFTAB5-2500 (for 2500U)

Description

DNA-Free Alkaline Phosphatase (TAB5) catalyzes the hydrolysis of phosphate monoesters but does not catalyze the hydrolysis of phosphate diesters or triesters. Alkaline Phosphatase (TAB5) is derived from psychrophilic bacteria, exhibiting high activity in the temperature range of 25-50℃. Due to its origin from psychrophilic bacteria, it has very low thermal stability and can be irreversibly inactivated by heat treatment at 65℃ for 15 minutes or 70℃ for 5 minutes.

Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to dephosphorylate 1 μg of pUC19 DNA digested with HindIII (5' overhangs), EcoRV (blunt ends), or PstI (3' overhangs) within 30 minutes at 37℃. Dephosphorylation is confirmed by the inhibition of >95% DNA recircularization in a self-ligation reaction (assessed by transforming E. coli competent cells with the ligation products).

Features

• Completely free from bacterial DNA.
• High activity within the temperature range of 25-50℃.
• Highly thermolabile, completely inactivated by heating at 65℃ for 15 minutes or 70℃ for 5 minutes.

Applications

• Removal of 5’ phosphate groups from DNA molecules.
• Pre-treatment of PCR products for sequencing when combined with Exonuclease I.

Storage

Store at -20℃ for up to 2 years. Avoid repeated freeze-thaw cycles.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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