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Cat. No.: EDN2-400 (for 4*100μl)

Cat. No.: EDN2-4k (for 4*1ml)

Description

Nucleic Acid Mass Spectrometry (NAMS) is a multi-PCR analysis and detection system developed based on MALDI-TOF MS mass spectrometry, which has emerged as a new molecular diagnostic platform following PCR and NGS in recent years. While fluorescence quantitative PCR detection is fast, its throughput is limited, unable to conveniently meet the detection requirements for dozens to hundreds of gene loci. High-throughput sequencing, although with extremely high throughput, entails high detection costs, long project detection cycles, and requires professional analysis and interpretation of the data, thus posing high technical barriers. NAMS, on the other hand, provides stable and accurate detection results at lower costs, meeting the demands for qualitative and quantitative detection of medium-throughput SNP loci and gene mutations. It also facilitates convenient DNA methylation and copy number variation (CNV) detection, highlighting its strong competitiveness in the field of genetic testing, thus becoming one of the main platforms driving rapid development in life sciences, especially in clinical diagnostics.

Nucleic Acid Mass Spectrometry primarily achieves nucleic acid detection through multiplex PCR, high-throughput chips, and time-of-flight mass spectrometry. This technology enables simultaneous detection of dozens or even hundreds of target markers in a single sample, with the capacity to process over 1000 samples per day.

The main steps of NAMS are as follows:

1. Polymerase Chain Reaction (PCR): Design upstream and downstream primers for the target loci, use sample DNA as a template, and perform PCR to obtain PCR product fragments containing the target loci.
2. Shrimp Alkaline Phosphatase (SAP) treatment: Add SAP to dephosphorylate the PCR products, eliminating residual dNTPs in the reaction system.
3. Single Nucleotide Extension Reaction: Add single nucleotide extension primers and a mixture of extension terminators (ddNTPs) to perform single nucleotide extension reactions.
4. Resin purification: Add desalting resin to desalt and purify the extension products, avoiding interference from salt ion peaks in the mass spectrometry results.
5. Sample-matrix binding: Spot the sample onto a chip with a matrix and perform mass spectrometry detection, resulting in different detection peak spectra for different gene fragments.

The core technology of NAMS lies in the single nucleotide extension technique, relying on ddNTPs as extension terminators. By analyzing the extension products formed during single nucleotide extension reactions, the aim is to analyze the nucleotide sequence.

Currently, the resolution of NAMS instruments on the market is around 10 Da. During single nucleotide extension, the molecular weight difference between regular ddATP and ddTTP is slightly less than 10 Da, making it impossible for the mass spectrometer to accurately identify A and T, leading to detection failure. This technical challenge has become one of the barriers limiting the clinical application of NAMS.

To overcome this technical barrier, SBS Genetech has dedicated efforts to research and development, finally overcoming the modification of ddNTPs for NAMS, and proudly introducing the specialized Termination-ddNTP (for MALDI-TOF MS) product! The molecular weight differences between the modified ddNTPs (for MALDI-TOF MS) are all greater than the resolution of the mass spectrometer (10 Da), enabling NAMS instruments to accurately identify A, T, C, and G, ensuring the accuracy of detection.

Features

• Nucleic Acid Mass Spectrometry Specific: This product is tailored for MALDI-TOF MS applications, ensuring accuracy in mass spectrometry detection with molecular weight differences between modified ddNTPs exceeding 10 Da!
• High Purity: HPLC>99%
• Stability: Exhibits excellent inter-batch stability, supported by a rigorous quality management system.
• Residue-free: Completely devoid of DNase and endonuclease residues, as well as any remnants of human or bacterial genomes.

Storage

Store at -20°C, effective for two years.

Precaution

• Can be thawed at room temperature. After thawing, it is advisable to store it in a cooler or in an ice bath. After use, it should be promptly stored at -20°C.
• During the experimental process, please use DNase-free and RNase-free tips and centrifuge tubes.
• This product is intended for scientific research by professionals only and is not intended for clinical diagnosis or treatment. It must not be used for food or drugs and must not be stored in residential settings.
• For your safety and health, please wear laboratory attire and disposable gloves when handling.

Related: ddNTP Mix (10mM each, Suitable for MALDI-MS)

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory