All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: EDN-100 (for 100μl)

Cat. No.: EDN-500 (for 500μl)

Cat. No.: EDN-2k (for 2ml)

Description

ddNTP Mix (10mM each, Suitable for MALDI-MS) is a mixture of ddATP, ddCTP, ddGTP, and ddTTP, each at a concentration of 10mM. This mixture is designed for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) analysis. It serves as a specialized set of mass-modified terminators for use in single-nucleotide extension reactions, enabling mass spectrometric analysis. Similar to "iPLEX Termination Mix" by Sequenom or Agena, this ddNTP Mix contains a modified ddTTP with a higher molecular weight compared to regular ddTTP. The distinct molecular weights after incorporation into DNA are approximately 271 Da, 247 Da, 287 Da, and 327 Da for ddATP, ddCTP, ddGTP, and the modified ddTTP, respectively. This facilitates high-throughput Single Nucleotide Polymorphism (SNP) analysis using MassArray mass spectrometry.

The discovery of several matrices capable of ionizing DNA and RNA has propelled Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) into a powerful platform for nucleic acid research. It can be used to investigate sequence variations such as mutations, Single Nucleotide Polymorphisms (SNPs), insertions/deletions, alternative splicing, copy number variations, gene expression, allele-specific expression, DNA methylation, post-transcriptional modifications in tRNA and rRNA, and more.

ddNTP (Dideoxynucleoside Triphosphate) differs from dNTP (deoxynucleoside triphosphate) as it lacks a 3'OH group due to the presence of a dideoxyribose moiety. This prevents the formation of phosphodiester bonds necessary for the addition of subsequent nucleotides during DNA synthesis or amplification, resulting in chain termination. Hence, ddNTPs are often referred to as "chain-terminating nucleotides."

In regular ddNTPs, the molecular weight differences among products after single-nucleotide extension are small, ranging from 9 to 40 Da, making high-throughput genotyping via mass spectrometry challenging. The modified ddTTP in this product has a larger molecular weight, ensuring differences of 15 Da or more among the products, allowing for genotyping based on the mass differences of the terminal bases. This innovation makes high-throughput genotyping feasible using mass spectrometry. The approximate mass differences (Da) for each terminator are presented in the table below:

Features

This product is specifically designed for MALDI-MS or MALDI-TOF-MS applications. It is of mass grade quality, with high purity (HPLC ≥ 99%), a concentration of 10mM for each ddNTP, pH of 7.4 ± 0.1, and it is free from nucleases, including DNase and RNase.

Storage

Store at -20°C, effective for two years.

Precaution

• Can be thawed at room temperature. After thawing, it is advisable to store it in a cooler or in an ice bath. After use, it should be promptly stored at -20°C.
• During the experimental process, please use DNase-free and RNase-free tips and centrifuge tubes.
• This product is intended for scientific research by professionals only and is not intended for clinical diagnosis or treatment. It must not be used for food or drugs and must not be stored in residential settings.
• For your safety and health, please wear laboratory attire and disposable gloves when handling.

Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.

Related: ddNTP Set (10mM, for MALDI-TOF MS)

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory