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Cat. No.: CCMN-0.2 (for 10mM×0.2ml)

Cat. No.: CCMN-25 (for 25mg)

Cat. No.: CCMN-100 (for 100mg)

Description

Curcumin (Diferuloylmethane), a natural phenolic compound, serves as a specific inhibitor of acetyltransferase for p300/CREB-binding protein. It suppresses the acetylation of histone/nonhistone proteins and histone acetyltransferase-dependent chromatin transcription. Curcumin demonstrates inhibitory effects on NF-κB and MAPKs, and boasts diverse pharmacological effects, including anti-inflammatory, antioxidant, antiproliferative, and antiangiogenic activities. Moreover, Curcumin induces stabilization of Nrf2 protein through Keap1 cysteine modification.

Basic Information

• Aliases: Diferuloylmethane
• Source: Curcuma longa L.
• Compound type: Phenylpropanoids > Lignans > Simple lignans
• Chemical Formula: C₂₁H₂₀O₆
• Molecular Weight: 368.38
• CAS Number: 458-37-7
• Purity: 98%, HPLC
• Solvent/Solubility: DMSO: 120 mg/mL (325.75 mM); Water: < 0.1 mg/mL (insoluble)
• Solution Preparation: Add 5mg to 1.36ml of DMSO, or add 3.68mg to 1ml of DMSO, to prepare a 10mM solution.

Signaling Pathway

NF-κB; MAPK

In Vitro Study

Curcumin exerts its chemopreventive effects partly through the activation of nuclear factor (erythroid-2 related) factor 2 (Nrf2) and its antioxidant and phase II detoxifying enzymes. It inhibits T47D cell growth, with IC50s of 25, 19, and 17.5 μM for 24, 48, and 72 h MTT assays respectively. The IC50s of the curcumin and silibinin mixture against T47D cells are 17.5, 15, and 12 μM for 24, 48, and 72 h exposure times, respectively. Curcumin induces apoptotic cell death in AGS and HT-29 cell lines within the range of 2.5-80 μM, with IC50 values of 21.9±0.1 and 40.7±0.5 μM, respectively. This apoptotic effect of curcumin requires caspase activities in both AGS and HT-29 cells. Furthermore, curcumin induces ER Ca2+ decline and mitochondrial Ca2+ overloading. It also induces G2/M cell cycle arrest of LNCaP and PC-3 cells in a dose-dependent manner, while upregulating the protein level of NF-kappaB inhibitor IkappaBalpha and downregulating protein levels of c-Jun and AR.

In Vivo Study

Curcumin (10 mg/kg, orally) significantly prevents a decrease in the percentage of sucrose consumption compared to rats exposed to chronic mild stress (CMS). Curcumin treatment significantly prevents an increase in TNF-α and IL-6 levels in stressed rats. At doses of 20 mg/kg (intraperitoneal), curcumin reduces the binding of p300/CREB-binding protein (CBP) at the brain-derived neurotrophic factor (BDNF) promoter. At 40 mg/kg, it reduces the binding of P300/CBP at the BDNF promoter. At 60 mg/kg, curcumin decreases the binding of all four proteins of p300/CBP and H3K9ac/H4K5ac at the BDNF promoter in chronic constriction injury (CCI) rats.

Clinical Trial

NCT04528212: Diabetes Mellitus, Type 2, Phase 4; NCT04044417: Periodontitis, Phase 4; NCT02532023: Migraine, Phase 4; NCT01052597: Type 2 Diabetes Mellitus|Cardiovascular Abnormalities, Phase 4; NCT03917784: PreDiabetes, Phase 4; NCT04205929: Bleeding|Implants|Breakthrough Bleeding, Phase 4; NCT00779493: Irritable Bowel Syndrome, Phase 4; NCT02494141: Polycystic Kidney, Autosomal Dominant, Phase 4; NCT01750359: Major Depression, Phase 4; NCT04032132: Periodontitis, Phase 4; NCT02298985: Chronic Schizophrenia, Phase 4; NCT01052025: Type 2 Diabetes|Pre-diabetes|Insulin Resistance|Cardiovascular Risk, Phase 4.

Storage

Store at -20°C for at least one year. The solid powder is stable at 4°C for at least one month. If dissolved in a non-DMSO solvent, it is recommended to aliquot and store at -80°C for up to six months.

Precautions

• This product may have a certain degree of toxicity to the human body. Please take appropriate precautions to avoid direct contact with the body or inhalation.
• This product is for scientific research use only by professionals and is not intended for clinical diagnosis or treatment, food, or pharmaceutical purposes. It should not be stored in ordinary residential areas.
• For your safety and health, please wear laboratory attire and disposable gloves while handling.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory