CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (One-Step)
$1,600.00 - $5,600.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: C13ALDO-96 (for 96T)
Cat. No.: C13ALDO-480 (for 480T)
Description
The Cas13a and crRNA form a functional complex that "slides" along the target nucleic acid sequence. Upon successful pairing, Cas13a is specifically activated and cleaves the surrounding reporter molecules. This kit combines isothermal amplification technology with Cas13a, featuring high sensitivity, high specificity, and a high signal-to-noise ratio. It is widely used in the field of molecular diagnostics for rapid and accurate detection of pathogens.
Kit Components
No. 1 | Item Detection Buffer (2X) | Size 1000 μl |
2 | Core Mix (4X) | 500 μl |
3 | Positive Control (10X) | 30 μl |
| (includes primer, crRNA, and DNA template) | ||
4 | Starter (10X) | 200 μl |
5 | Cas13a Protein (10 μM) | 20 μl |
6 | Cas13a Diluent Buffer | 100 μl |
7 | NTP Mix (25 mM each) | 100 μl |
8 | T7 RNA Polymerase (40X) | 50 μl |
Additional Required Materials
- Fluorometer for reading FAM signals (e.g., qPCR instrument)
- Pipettes
- Nuclease-free water
- Target sequence-specific primers for isothermal amplification
- crRNA/gRNA: Forms a functional complex with LbaCas12a and is specifically activated by the target sequence. (LbaCas12a crRNA scaffold sequence: 5’-UAAUUUCUACUAAGUGUAGAU-3’)
Related:
- CRISPR Cas12a-RPA DNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas12a-RPA Lyophilized DNA Detection Kit (Two-Step)
- CRISPR Cas12a-RPA DNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (Two-Step)
- CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- CRISPR Cas12a-RPA RNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas12a-RPA Lyophilized RNA Detection Kit (Two-Step)
- CRISPR Cas12a-RPA RNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (Two-Step)
- CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- Lateral Flow Assay for CRISPR SHERLOCK
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