Cas14a1 is a type of endonuclease that acts as a restriction enzyme, specifically binding and cleaving target single-stranded DNA (ssDNA) under the guidance of tracrRNA:crRNA (or sgRNA), and it does not require a PAM site.

Compared to other Cas proteins, Cas14 proteins generally have a smaller molecular weight (400-700aa). Similar to Cas12, Cas14a1 can bind to target nucleic acids and activate its ssDNA endonuclease activity, making it applicable for molecular detection of target nucleic acids. Unlike Cas12, Cas14a1 can only bind to ssDNA targets. Therefore, target nucleic acids that have undergone amplification enrichment need to be treated with T7 endonuclease, and one of the amplification primers needs to be phosphorothioate-modified to ensure that T7 endonuclease cleaves only one strand, leaving the ssDNA target strand for Cas14a1-mediated molecular detection.

The lyophilized version of Cas14a1 can be transported at room temperature, saving the high cost of dry ice transportation.

The minimum shelf life is 18 months at 2-28°C for Cas14a1 and 1 year at -20°C for 10×Buffer.

Instruction: Protocol

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Related: 

• Synthetic sgRNAs
• Cas12a Nuclease (Lyophilized)
• Cas12b Nuclease (Lyophilized)
• Cas13a Nuclease (Lyophilized)
• Cas14a1 Nuclease (Lyophilized)

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