Cat. No.: BSPGF-16k (for 16KU)

Cat. No.: BSTLB (for Bst DNA/RNA Lyo Buffer)

For better isothermal amplification of RNA, please see Bst DNA/RNA Polymerase.

For custom-ready mastermix (including visualization dye, probes, primers, etc), please email your requirements to tech@sbsbio.com.

The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.

Description

Bst Polymerase (glycerol-free) was derived from Bacillus stearothermophilus DNA polymerase I. Its 5'- 3' and 3'-5' exonuclease activity was removed by genetic engineering, while the 5'- 3' polymerase activity was retained. Compared with wild-type Bst DNA polymerase (large fragment) and Bst DNA Polymerase, Bst Polymerase has better isothermal amplification activity and stronger reverse transcription activity. Single enzyme system reaction can be realized in the isothermal amplification experiment with RNA as a template. Bst Polymerase has good reverse transcription activity at 60-65°C, which can effectively solve the reverse transcription of the RNA template with secondary complex structure, while Bst DNA Polymerase and wild-type Bst DNA polymerase (large fragment) do not have this activity. This product has a high concentration (32U/μl) and is glycerol-free, which can be used to establish a freeze-drying system.

Contents

• DNA and RNA isothermal amplification in a single enzyme reaction
• GC-rich rapid sequencing
• Rapid sequencing of micro-template DNA

Storage Conditions

Store the components at -20°C. Avoid multiple freeze-thaw cycles.

Bst DNA/RNA Lyo Buffer (Optional)

Bst DNA/RNA Lyo Buffer is a special lyophilization buffer system for Bst DNA/RNA Polymerase with 2.5 × concentration, including reaction buffer salt, Mg²⁺ and lyophilization excipient. dNTP and Bst DNA/RNA Polymerase (glycerol-free) can be added directly when preparing the freeze-drying system. With this optimized buffer, there is no need to optimize the conditions of freeze-drying protective agent.

Note: Bst DNA/RNA Polymerase (glycerol-free) doesn't contain Bst DNA/RNA Lyo Buffer. If Bst DNA/RNA Lyo Buffer is required, please include it when placing the order.

Instruction: Protocol (Bst Polymerase, glycerol-free), Protocol (Bst DNA/RNA Lyo Buffer)

Related: 

• Bst DNA Polymerase Large Fragment
• Bst DNA Polymerase (comparable to 2.0)
• Bst Polymerase (comparable to 3.0)
• Bst Polymerase (glycerol-free)
• Bst DNA/RNA Polymerase (with superior reverse transcription activity) 
• Bst DNA/RNA Polymerase (glycerol-free)
• Bst P DNA/RNA Polymerase (glycerol-free) (with superior reverse transcription activity & hot-start property) 

Customized Lyophilized Microbead Service

Looking for a personalized solution for your research needs? 

Look no further than our customized lyophilized microbead services! We offer lyophilized microbeads with customized primers and reaction volumes of 20-100 μl per bead, ensuring that your experiments are optimized to your exact specifications. Don't settle for a one-size-fits-all approach - Contact us today to learn more about how our customized lyophilized bead services can take your research to the next level!

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals