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Cat. No.: BSAIEK-96 (for 96T)

Cat. No.: BSAIEK-480 (for 480T)

Description

This product utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) to detect the BsaI content in samples. BsaI-specific antibodies are pre-coated onto the microwell plate. During use, standard samples and test samples are added separately to the reaction wells coated with the specific antibodies. BsaI in the samples will bind to the antibodies on the microwell plate. After incubation, washing is performed to remove unbound substances from the microwell plate. BsaI detection antibodies (HRP-labeled) are then added to form a complex of antibody-antigen-HRP-labeled antibody. TMB is added for color development, and the color intensity is proportional to the concentration of BsaI. The residual level of BsaI in the samples is determined by measuring the color development of BsaI standards.

• Detection Range: 0.188~12 ng/ml
• Quantification Limit: 0.188 ng/ml
• Detection Limit: 0.094 ng/ml

Storage

Store at 2-8°C. When unopened, the kit can be stored stably at 2-8°C for 12 months. Avoid exposure to light.

Precautions

• This product is for research use only and is not intended for clinical diagnosis or treatment.
• Reagents should be stored according to the conditions indicated on the label, and both reagents and samples should be equilibrated to room temperature before use.
• Prior to using the pre-coated enzyme plate, it should be equilibrated to room temperature and then the packaging bag should be opened to remove the required amount of strips. Any remaining strips should be promptly returned to the packaging, resealed, and stored at 2-8°C. Remaining reagents should also be resealed and stored according to the conditions indicated on the label.
• BsaI standard and BsaI detection antibody (HRP-labeled) solutions should be thoroughly mixed before use.
• Any residual liquid in the wells during the washing process should be gently blotted dry on clean, lint-free paper. Overdrying of the wells after blotting should be avoided to prevent improper washing, which may result in higher CV values for duplicate wells or higher absorbance values for blank (0 ng/ml) wells, affecting result accuracy.
• The TMB substrate should be a colorless, transparent liquid. If it appears light blue, it indicates contamination and is unsuitable for subsequent analysis.
• Samples with extreme pH values (<5.0 or >8.5), or containing high salt, high polysaccharide, high urea, high organic solvent, or high detergent concentrations, may result in lower recovery rates.
• Due to the complexity of biological sample matrices, it is recommended to include positive control test samples in each assay to ensure assay accuracy, and to use duplicate wells for testing.

Safety Precautions

• The termination solution is an acidic solution, so safety precautions should be taken when using it.
• Operators should wear personal protective equipment such as lab coats, gloves, masks, and safety goggles.

Related:

• Benzonase ELISA Kit
• BsaI ELISA Kit
• XhoI ELISA Kit
• XbaI ELISA Kit
• BsmBI ELISA Kit

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory