Bovine Serum Albumin (Fatty Acid & IgG Free)
$58.00 - $578.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: BSAFIF-5 (for 5g)
Cat. No.: BSAFIF-20 (for 20g)
Cat. No.: BSAFIF-100 (for 100g)
Description
BSA stands for Bovine Serum Albumin, a meticulously purified and lyophilized protein that finds versatile utility across various scientific applications. Whether utilized in Western blots, enzyme systems, protein standards, hybridizations, or protease-sensitive immunoassays, BSA consistently delivers reliable performance. Moreover, it serves as an excellent nutrient in cell culture media, exhibiting minimal interference and backgrounds, making it an optimal choice for standard molecular biology experiments.
Comprising approximately 583 amino acid residues and devoid of carbohydrates, BSA protein exists as a single polypeptide chain with a molecular weight of around 66kDa. Its designation as "Fraction V" harkens back to its origin in the fifth fraction of the original Cohn purification process, utilizing cold ethanol precipitation. This nomenclature persists despite advancements in purification methods.
For tailored applications requiring enhanced formulation, Fatty Acid & IgG Free BSA offers the flexibility to incorporate specific fatty acids or mitigate interference from IgG, thereby optimizing cell culture system performance. Particularly beneficial for fatty acid-sensitive cell cultures like CHO, Vero, and MDCK cells, as well as delicate research assays, this variant ensures purity by being protease-free and certified free from Bovine Viral Diarrhoea (BVD).
The absence of IgG in this BSA variant is particularly advantageous, as it prevents unwanted alterations in experimental data and reduces non-specific binding in critical immunoassays. In processes such as monoclonal antibody production, the elimination of IgG significantly improves purification yields of the target protein, ensuring the integrity and accuracy of scientific outcomes.
Application
Bovine Serum Albumin (BSA) finds widespread use as a carrier protein for antibody dilution and as a universal blocking agent in immunoassays and immunodetection protocols. Fatty Acid & IgG Free BSA is specifically designed for fatty acid and IgG-sensitive applications, including ELISA, RIA, EIA, Fluorescent and Chemiluminescent assays, serving as a stabilizer for conjugates or enzymes, as well as in hybridization and nucleic acid-based assays. It is also invaluable in maintaining optimal conditions for fatty acid and IgG-sensitive cell culture systems.
Basic Information
| Parameter | Value |
|---|---|
Non-Esterified Fatty Acid | <0.01% |
IgG (Turbidimetric Analysis) | Not Detected |
ENDOTOXIN (LAL) | <0.01 EU/mg |
PROTEASE (Enzymatic Assay) | Negative |
PROTEIN (Dry Basis) | ≥99% |
pH (10% Solution) | ~7.2 |
PURITY (Agarose Gel) | ≥99% |
E. COLI BY MPN (USFDA-BAM) | <3 MPN/g |
MOISTURE (AOAC 930.15) | <5.0% |
SOLUBILITY (6% W/V IN DI H2O) | Soluble |
ASH (AOAC 923.03) | <2.0% |
HEAVY METALS (AOAC 984.27) | < 10 ppm |
SALMONELLA (BACGene) | Absent |
MICROBIAL CONTENT (AOAC 990.12) | <10 cfu/g |
MYCOPLASMA (Culture) | Negative |
BVD (Virus Isolation) | Negative |
PATHOGEN INDUCING CYTOPATHY | Negative |
Storage
Store at 4°C.
Notes
- This product is intended for scientific research by professionals only and should not be used for clinical diagnosis or treatment, nor for food or drug purposes. It should not be stored in residential areas.
- For your safety and health, please wear laboratory attire and disposable gloves while handling.
Only for research and not intended for treatment of humans or animals
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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory
