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Cat. No.: BRIMB-10 (for 10T)

Cat. No.: BRIMB-50 (for 50T)

Cat. No.: BRIMB-200 (for 200T)

Description

Blood RNA Isolation Kit with Magnetic Beads is designed for the safe, rapid, convenient, stable, and high-quality extraction of total RNA from blood using magnetic beads coated with a novel nucleic acid purification medium. The extracted RNA includes both high molecular weight RNA and small RNA (<200nt), often referred to as total RNA, and can be used for various conventional molecular biology and biochemical analyses.

The RNA extracted using this kit can be used for downstream applications such as reverse transcription, RT-PCR, qPCR, Northern blotting, dot blotting, mRNA purification, in vitro translation, RNase protection assays, cDNA cloning, gene expression microarray analysis, and high-throughput sequencing, which require high-quality RNA.

RNA can be categorized by length into long RNA (>200nt) and small RNA (<200nt). Long RNA can be further divided into long noncoding RNA (lncRNA) and mRNA based on whether they encode proteins or peptides. Small RNA includes noncoding RNAs such as 5.8S rRNA, 5S rRNA, tRNA, microRNA (miRNA), siRNA, piRNA, tsRNA, and srRNA.

Components

• RNA Magnetic Beads
• Lysis Buffer
• Binding Buffer
• Washing Buffer I
• Washing Buffer II
• Elution Buffer
• DNase
• Reaction Buffer (10X)

Extraction Process

The basic extraction process involves rapid lysis of the sample in a lysis buffer, releasing total RNA. The RNA binds specifically to the magnetic beads, followed by three washes to remove nonspecific proteins, salts, and other impurities. Finally, the RNA is eluted with an elution buffer to obtain high-purity RNA.

Features

• Safe Usage: This kit uses magnetic beads coated with a special nucleic acid purification medium to separate and purify RNA, effectively avoiding the use of toxic and harmful organic reagents like phenol and chloroform, which are used in traditional TRIzol LS extraction methods.
• Quick and Convenient: The kit does not require the use of erythrocyte lysis buffer to remove anucleated red blood cells, thus preventing RNA degradation. The magnetic bead purification method eliminates the need for cumbersome RNA precipitation steps, making the extraction process only 15-20 minutes long. Compared to traditional TRIzol LS extraction methods, this kit significantly simplifies the process, shortens the extraction time, and reduces the risk of RNA degradation. The steps and time required are similar to those of comparable column purification products from abroad.
• High Yield and Purity: The kit consistently produces high yields of high-purity RNA. Pure RNA typically has an A260/A280 ratio of about 2.0, though readings above 2.0 are common on many instruments, while readings below 1.9 indicate contamination by proteins, DNA, or phenols. Pure RNA has an A260/A230 ratio of about 2.0 or slightly higher, with readings below 1.9 suggesting contamination by carbohydrates, guanidine salts, peptides, or phenols. Tests show that RNA extracted with this kit usually has an A260/A280 ratio of 2.0-2.2 and an A260/A230 ratio of 1.9-2.1.

Storage

• Store at room temperature for up to one year, except for DNase and Reaction Buffer (10X), which should be stored at -20ºC for one year.
• RNA Magnetic Beads can be stored at 4ºC for longer-term storage if not used frequently.

Precautions

• Before first use, add the specified amount of anhydrous ethanol to the Binding Buffer and Washing Buffer II as indicated on the instruction manual or bottle label, mix well, and mark the bottle.
• A magnetic separation device is required. You can procure one from our company if needed.
• If DNase provided in the kit is not used, the extracted RNA may contain small amounts of DNA. For experiments sensitive to DNA residues, add an appropriate amount of DNase to the magnetic beads after step 4 in the instructions for digestion. Refer to the instruction manual for details.
• Use the recommended amount of blood for RNA extraction to prevent RNA magnetic beads from clumping and ensure thorough genomic DNA digestion. If genomic DNA digestion is insufficient, increase the DNase amount or extend the incubation time.
• All reagents and consumables provided in the kit are RNase-free. Handle carefully to avoid contamination. Ensure all self-prepared reagents and consumables are RNase-free. To prevent RNase contamination, consider soaking in 0.01% DEPC water overnight, then autoclaving and drying. Avoid breathing or talking over samples or kit consumables. Wearing a disposable mask is recommended.
• Perform all operations at room temperature, with no need for ice baths. Conduct all centrifugations at room temperature.
• Reuse the waste collection tube multiple times in a single extraction; do not discard it midway.
• Binding Buffer and Washing Buffer contain ethanol. Tighten the bottle caps after use to prevent evaporation and store and handle according to flammable reagent guidelines.
• This product is for professional scientific research use only. It is not intended for clinical diagnosis or treatment, nor for use in food or medicine. Do not store it in common household environments.
• For your safety and health, wear a lab coat and disposable gloves during handling.

Only for research and not intended for treatment of humans or animals

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