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Cat. No.: AMMB-0.5 (for 0.5ml)

Cat. No.: AMMB-2 (for 2ml)

Description

Anti-MBP Magnetic Beads, also known as Anti-Maltose Binding Protein (MBP) Magnetic Beads, Anti-MBP Beads, Anti-MBP Immunomagnetic Beads, or MBP Antibody Magnetic Beads, are covalently coupled with high-quality MBP-tag mouse monoclonal antibodies and nanoscale amino magnetic beads. They can specifically bind to proteins containing MBP tags in samples such as cell or microbial lysates, serum, and ascites. These beads are used for immunoprecipitation (IP), co-immunoprecipitation (Co-IP), or purification of fusion proteins or protein complexes carrying MBP tags.

MBP tags (Maltose Binding Protein tags), along with other common tags such as His tags, Flag tags, Myc tags, HA tags, and GST tags, are commonly used labels on expression vectors. Fusion expression with these tags facilitates convenient detection of the target protein and proteins that interact with it. Moreover, it is also convenient for the purification of the target protein.

Maltose Binding Protein (MBP), encoded by the malE gene, is part of the Escherichia coli (E. coli) maltose/maltodextrin system. It is responsible for the uptake, breakdown, and metabolism of maltodextrin, with a molecular weight of approximately 42 kDa. The MBP tag, fused to the N- or C-terminus of a protein (usually N-terminus), has several advantages, including improving the solubility of exogenous proteins, especially those accumulating in insoluble forms like inclusion bodies (IBs). It has a wide applicability, working in different hosts such as E. coli and yeast, enhancing the expression levels of the target protein. Additionally, it promotes the correct folding of proteins. If there are specific proteinase recognition sites between the MBP tag and the target protein, such as PreScission Protease, TEV Protease, or Thrombin, the MBP tag can be cleaved using the corresponding proteinase. The MBP tag is highly specific, making purification convenient and gentle. With the MBP tag as a labeled protein, subsequent detection, localization, and functional studies of the target protein can be conducted using the MBP antibody or Anti-MBP Magnetic Beads, facilitating protein purification, immunoprecipitation, or co-immunoprecipitation. Due to these advantages, the MBP tag has been widely used in various aspects of research, including protein expression, purification, identification, interaction, and functionality. While general purification media for MBP-tagged proteins are used for the purification of MBP-tagged proteins, Anti-MBP Magnetic Beads offer a simpler and more convenient solution for applications such as the minimal purification or immunoprecipitation of fusion proteins or protein complexes with MBP tags.

Anti-MBP Magnetic Beads can specifically bind to MBP-tagged fusion proteins and can be conveniently applied in immunoprecipitation or purification experiments involving fusion proteins or protein complexes with MBP tags using magnetic separation devices such as magnetic racks.

Features

• This product exhibits strong specificity and high binding capacity to the target protein. Compared to most similar products domestically and internationally, this product has a high antibody binding density, displaying strong specificity for proteins with MBP tags. Additionally, the magnetic beads in this product have a small particle size, reducing the likelihood of nonspecific adsorption. Each milliliter of magnetic bead suspension contains approximately 10 mg of magnetic beads, with no less than 0.6 mg of MBP antibody. Typically, it can bind to no less than 0.6 mg of MBP-tagged fusion protein, and the specific maximum binding capacity depends on the size of the target protein and its molecular weight. For immunoprecipitation experiments, only 10-20 microliters of magnetic bead suspension are usually required for efficient use with 500 microliters of sample.
• This product can bind to various forms of MBP-tagged proteins, specifically binding to N-terminal MBP fusion proteins (MBP-Protein) and C-terminal MBP fusion proteins (Protein-MBP).
• The binding process with the target protein is rapid. Utilizing nanoscale beads (~200 nm) with an ultra-large surface area facilitates the rapid and effective binding of antibodies and antigens. Typically, the antigen adsorption process can be completed within 30 minutes, and the immunoprecipitation of the target protein can be achieved within 60 minutes. Shortening the operation time helps to avoid degradation or denaturation of the target protein during prolonged operations, ensuring the full preservation of the target protein's activity.
• This product offers a variety of elution methods. Depending on the structure, biological function, and subsequent application requirements of the target protein, various elution methods can be employed, including elution with SDS-PAGE sample buffer and acidic elution solutions.

Storage

Store at -20ºC, valid for two years. Store at 4ºC, valid for at least one month.

Note

• Due to the relatively large molecular weight of the MBP tag itself, approximately 42 kDa, and the increased molecular weight when fused with the target gene, the complexity of the fused protein's structure may affect the recognition of the MBP tag by Anti-MBP Magnetic Beads. It is recommended to perform a preliminary immunoprecipitation experiment for effect validation. Adjustments to the lysis conditions may be necessary if needed.
• Due to the strong binding affinity between the MBP tag and the MBP antibody, the effectiveness of acidic elution may be relatively poor. It is recommended to prioritize the use of the SDS-PAGE sample buffer elution method.
• Maintain the pH of this product between 6-8, avoid high-speed centrifugation, and prevent drying. Do not leave the magnetic beads in the magnetic field for an extended period, as this may lead to bead aggregation.
• Thoroughly resuspend the product before use by gently inverting it several times. Mixing should be done gently, avoiding vigorous vortexing or shaking to prevent antibody denaturation, etc.
• During immunoprecipitation or purification, it is advisable to set up positive and negative control groups.
• After collecting protein samples, complete the purification work as soon as possible, and always keep them at 4ºC or on ice to slow down protein degradation or denaturation. To effectively inhibit protein degradation, add an appropriate amount of a protease inhibitor mixture to the protein sample.
• When using instruments such as a vacuum pump to aspirate the supernatant, pay attention to the suction strength to avoid overly strong suction that may aspirate aggregated magnetic beads.
• During acidic elution, bead aggregation may occur, which is a normal phenomenon and does not affect the normal use of the magnetic beads. The addition of 0.1% non-ionic detergent (such as Triton X-100, Tween-20, or NP-40) can effectively prevent bead aggregation without affecting the antibody binding efficiency of the magnetic beads.
• High concentrations of DTT, mercaptoethanol, guanidine hydrochloride, etc., may have some impact on the binding of this product to tagged proteins.
• This product is for scientific research use by professionals only and is not intended for clinical diagnosis or treatment. It should not be used for food or drugs and should not be stored in ordinary residential areas.
• For your safety and health, wear laboratory attire and disposable gloves during operations.

Only for research and not intended for treatment of humans or animals

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