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Cat. No.: AGMB-0.5 (for 0.5ml)

Cat. No.: AGMB-2 (for 2ml)

Description

Anti-GFP Magnetic Beads, also known as Anti-GFP Immunomagnetic Beads or GFP Antibody Magnetic Beads, are covalently coupled with high-quality GFP mouse monoclonal antibodies and nano-sized amino magnetic beads. They can specifically bind to proteins containing the GFP tag in lysates, serum, ascites, and other samples derived from animals, plants, or microorganisms. These beads are used for immunoprecipitation (IP), co-immunoprecipitation (Co-IP), or purification of fusion proteins or protein complexes carrying the GFP tag.

GFP tag, Flag tag, Myc tag, HA tag, His tag, GST tag, and other commonly used tags are frequently employed in expression vectors. Fusion expression with these tags allows convenient detection of the target protein and its interacting partners and facilitates the purification of the target protein.

GFP (green fluorescent protein) was initially discovered by Shimomura, Chalfie, and Tsien in 1962 in the jellyfish Aequorea victoria. It emits green fluorescence when excited by ultraviolet or blue light. GFP or its variant EGFP (enhanced green fluorescent protein) fused with the target protein is widely used in studies involving gene expression regulation, transgenic functions, expression, distribution, migration of the target protein within cells, high-throughput drug screening, and other aspects. The advantages of the GFP tag include easy observation and suitability for studying protein localization within cells. It can be directly observed in living cells using fluorescence microscopy without the need for tissue disruption, substrates, or antibodies. The emitted green fluorescence allows real-time monitoring of the expression of the target protein. GFP exhibits stable fluorescence properties, and other cellular components do not interfere with the detection of GFP-tagged proteins, making it a rapid, simple, highly sensitive, and reproducible detection method. Therefore, GFP is regarded as a probe for live cell imaging. However, the GFP tag has a relatively large molecular weight of approximately 25 kDa, which may potentially affect the function of the target protein when fused.

Anti-GFP Magnetic Beads can specifically bind to fusion proteins carrying the GFP or EGFP tag. They can be conveniently applied in immunoprecipitation, purification, and other experiments involving fusion proteins or protein complexes with the GFP tag, using magnetic separation devices such as magnetic racks.

Features

• This product has strong specificity and high binding capacity to its target protein. Compared to most similar products domestically and abroad, this product has a high density of antibody binding. It exhibits strong specificity towards proteins tagged with GFP and the magnetic beads of this product are of fine granularity, minimizing non-specific adsorption. Each milliliter of the magnetic bead suspension contains approximately 10mg of beads, and no less than 0.6mg of GFP antibodies. It can typically bind no less than 0.6mg of GFP-tagged fusion proteins, with the exact maximum binding capacity being related to the molecular weight of the tagged protein. For every 500µl sample, typically only 10-20µl of magnetic bead suspension is needed for efficient immunoprecipitation.
• This product can bind to various forms of GFP-tagged proteins. It can specifically bind to N-terminal GFP fusion proteins (GFP-Protein) and C-terminal GFP fusion proteins (Protein-GFP).
• This product binds target proteins quickly. It employs nanoscale magnetic beads (~200nm) which have an exceedingly large surface area, facilitating rapid and effective antibody-antigen binding. Typically, the antigen adsorption process can be completed in 10 minutes and the target protein immunoprecipitation operation in 30 minutes. Shortening the operation time effectively prevents degradation or denaturation of the target protein over prolonged periods, ensuring the activity of the target protein.
• Multiple elution methods can be chosen for this product. Depending on the structure of the target protein, its biological function, and subsequent application requirements, various elution methods can be used, including SDS-PAGE sample buffer and acidic elution solutions.

Storage

Stored at -20ºC, effective for two years. Stored at 4ºC, effective for at least three months.

Only for research and not intended for treatment of humans or animals

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