Annexin V-FITC Apoptosis Detection Kit
$390.00 - $530.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: AVFA-50 (for 50T)
Cat. No.: AVFA-100 (for 100T)
Description
Annexin V-FITC Apoptosis Detection Kit is a cell apoptosis assay kit that uses FITC-labeled recombinant human Annexin V to detect phosphatidylserine, which appears on the cell membrane surface during apoptosis. It can be used with flow cytometers, fluorescence microscopes, or other fluorescence detection devices.
Annexins are a widely distributed group of calcium-dependent phospholipid-binding proteins found in the cytoplasm of eukaryotic cells, involved in intracellular signaling. However, only Annexin V has been reported to regulate the activity of certain PKC isoforms.
Annexin V selectively binds to phosphatidylserine (PS). Phosphatidylserine is primarily located on the inner side of the cell membrane, adjacent to the cytoplasm. During the early stages of apoptosis, various types of cells translocate phosphatidylserine to the outer surface of the cell membrane, facing away from the cytoplasm. The exposure of phosphatidylserine on the cell surface promotes clotting and inflammatory reactions. Annexin V, when bound to the phosphatidylserine exposed on the cell surface, can inhibit the procoagulant and proinflammatory activities of phosphatidylserine.
By using Annexin V labeled with a green fluorescent probe, FITC, known as Annexin V-FITC, the translocation of phosphatidylserine, an important feature of cell apoptosis, can be easily and directly detected using a flow cytometer or fluorescence microscope.
This assay kit also provides propidium iodide (PI) staining solution, which stains necrotic cells or cells with compromised membrane integrity in the late stages of apoptosis, exhibiting red fluorescence. In the case of necrotic cells, Annexin V-FITC can enter the cytoplasm due to the loss of cell membrane integrity and bind to phosphatidylserine on the inner side of the cell membrane, resulting in green fluorescence in necrotic cells as well.
Additional Notes
- Although Annexin V-FITC has been tested and found to have no significant impact on its detection performance after five freeze-thaw cycles, it is recommended to store it at 4°C within 3-6 months for optimal results and to avoid repeated freeze-thaw cycles.
- Contamination with bacteria or fungi can severely affect the detection performance.
- After staining, it is advisable to perform the detection as soon as possible, as prolonged time may increase the number of apoptotic or necrotic cells.
- If trypsin is used during the cell collection process, efforts should be made to remove any residual trypsin. Residual trypsin can digest and degrade Annexin V-FITC, resulting in staining failure.
- Fluorescent substances are prone to photobleaching. When conducting fluorescence observations, try to shorten the observation time and also store and handle the samples in a light-protected manner.
- When using a flow cytometer for detection, if there are too many PI false-positive cells observed during Annexin V-FITC staining, and adjusting the related settings and parameters does not improve the situation, Annexin V-FITC can be diluted 3-10 times with PBS before detection. This usually effectively reduces false-positive staining of necrotic cells.
- PBS (phosphate-buffered saline) needs to be prepared separately.
- This product is intended for scientific research by professionals only and should not be used for clinical diagnosis or treatment, food or drug purposes, or stored in regular residential areas.
- For your safety and health, please wear appropriate laboratory attire and disposable gloves when handling.
Storage
Store at 4°C for up to six months or at -20°C for up to one year. Annexin V-FITC and propidium iodide staining solution should be stored in light-protected conditions. For long-term storage, propidium iodide staining solution can be aliquoted and stored at -20°C.
Related: 7-AAD Cell Viability Assay Kit
Only for research and not intended for treatment of humans or animals
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