Annexin V-APC/7-AAD Apoptosis Detection Kit
$496.00 - $788.00
$985.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: AVAP7-50 (for 50T)
Cat. No.: AVAP7-100 (for 100T)
Description
In normal cells, phosphatidylserine (PS) is distributed only on the inner side of the lipid bilayer of the cell membrane. However, in the early stages of apoptosis, phosphatidylserine (PS) flips from the inner side to the outer side of the lipid bilayer. Annexin V is a 35-36 kDa Ca²⁺-dependent phospholipid-binding protein with a high affinity for phosphatidylserine. Therefore, it can bind to the cell membrane of early apoptotic cells through the exposed phosphatidylserine on the outer side of the cell. Consequently, Annexin V is used as a sensitive marker for detecting early apoptosis. When Annexin V is labeled with the fluorescent dye APC, the labeled Annexin V serves as a fluorescent probe. Apoptosis can be detected using a fluorescence microscope or flow cytometer.
7-AAD (7-amino-actinomycin D) is a nucleic acid dye that cannot pass through intact cell membranes. As cells undergo apoptosis and cell death, the permeability of the membrane to 7-AAD gradually increases. Combined with the controlled degradation of DNA during apoptosis, 7-AAD emits bright red fluorescence when excited by light of the appropriate wavelength. By measuring the intensity of 7-AAD staining of DNA, cells can be classified into three groups: 7-AAD strong (dead cells), 7-AAD weak (apoptotic cells), and 7-AAD negative (normal viable cells). 7-AAD has fluorescence characteristics similar to PI but with a narrower emission spectrum, causing less interference with other detection channels. It is an ideal substitute for PI in multicolor fluorescence analysis and can be used in conjunction with Annexin V-APC.
This kit is suitable for detecting apoptosis in cultured cells (not recommended for detecting tissue samples).
Components
- AnnexinV-APC
- 7-AAD Dye
- Binding Buffer
Additional Notes
- It is recommended to aliquot and store Annexin V-APC components at -20°C, protected from light, and avoid repeated freeze-thaw cycles.
- Store the Binding Buffer component at 4°C when not in use and protect it from light.
- 7-AAD is a potential carcinogen; take protective measures such as wearing protective clothing and gloves when handling it.
- Please centrifuge micro-reagents to collect the liquid before use.
- This kit is suitable for detecting live cells. When using a flow cytometer, the cell count should not be lower than 1x105. It is not recommended for detecting tissue samples.
- Suspension culture cells are recommended. If using adherent cells, digest with trypsin without EDTA, as improper digestion may cause false positives. Using a cell scraper can cause cell clumping, affecting detection. Cells digested with trypsin can be stored in PBS containing 2% BSA to prevent further damage.
- Fixation of cells may cause fluorescence quenching; please do not fix the samples.
- Due to variability in cell types, apoptosis inducers, and detection instruments, fluorescence compensation for flow cytometry will differ. Therefore, it is recommended to use cells treated with apoptosis inducers as controls for fluorescence compensation adjustment in each experiment.
Storage
Store at 2-8°C for 12 months, protected from light.
Related:
- Annexin V-APC/SYTOX Green Apoptosis Detection Kit
- Annexin V-sFlour647 Apoptosis/PI Detection Kit
- Annexin V-sFlour488 Apoptosis/PI Detection Kit
- Annexin V-APC/PI Apoptosis Detection Kit
- Annexin V-APC/7-AAD Apoptosis Detection Kit
- Annexin V-PE Apoptosis Detection Kit
- Annexin V-FITC/PI Apoptosis Detection Kit
- Annexin V-EGFP/PI Apoptosis Detection Kit
Only for research and not intended for treatment of humans or animals
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