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Cat. No.: AM1IM2IC-20 (for 20 ea)

Aflatoxin M1&M2 Immunoaffinity Column selectively adsorbs aflatoxin M1 and M2 from the sample solution, thereby providing highly targeted purification of the sample. The sample solution after column purification can be directly used for HPLC analysis. Combining the affinity column with HPLC enables rapid determination, improving the signal-to-noise ratio and enhancing the accuracy of the detection method.

Principle

The assay is based on antigen-antibody reaction. Antibodies are immobilized within the column. After extraction and centrifugation, the sample slowly passes through the aflatoxin M1 and M2 immunoaffinity column. Within the column, the toxins bind to the antibodies. Subsequently, washing removes any unbound substances. Aflatoxin M1 and M2 are then eluted with methanol, dried with nitrogen, and reconstituted before being injected into the analytical instrument for detection.

Precautions

• Before use, the immunoaffinity column must be equilibrated to room temperature (22-25°C).
• Store the affinity column at 2-8°C; do not freeze.
• Do not use immunoaffinity columns that have exceeded their expiration date.
• Sample volume: The sample volume can be appropriately increased or decreased according to requirements, but ensure that the volume of the extraction solution is adjusted accordingly.
• Column capacity: The total column capacity for aflatoxin M1 and M2 is 200 ng. If the content of the toxin to be detected in the sample divided by the dilution factor exceeds the column capacity, the volume of the sample solution should be appropriately reduced.
• pH: The pH of the sample solution loaded onto the affinity column should be between 6 and 8. If it deviates from this range, adjust the pH with hydrochloric acid or sodium hydroxide.
• Consistency between solvents and mobile phases during machine detection can eliminate the solvent effect.
• Aflatoxin M1 and M2 can be carcinogenic, so gloves should be worn during operation.
• It is advisable to soak used containers and solutions containing aflatoxin M1 and M2 overnight in a sodium hypochlorite solution (5% V/V).
• When powdered milk samples are difficult to dissolve, they can be left to stand at 50°C for 30 minutes. If they still do not dissolve, the proportion of water used for dissolution should be increased appropriately.

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Only for research and not intended for treatment of humans or animals

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