9N (exo-) DNA Polymerase
$585.00 - $4,680.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: 9NE-500 (for 500U)
Cat. No.: 9NE-5k (for 5KU)
Description
9N (exo-) DNA Polymerase is a mutant of 9N DNA polymerase. It has a strong ability to incorporate and modify substrates, such as ddNTP, rNTP, and acyNTP. Therefore, it is suitable for ddNTP or acyNTP chain termination sequencing, or SNP analysis. The enzyme was derived from E.Coli strain, which contains the genetically engineered 9°N (D141A/E143A/A485L) DNA Polymerase gene cloned from Thermococcus species 9 ° N-7.
Components
- 9N (exo-) DNA Polymerase (2 U/μl): 250 μl
- 10×HG PCR Buffer 6: 1 ml×2
Features
- Improved the incorporation ability of modified nucleotides
- Chain termination of ddNTP or acyNTP was used for sequencing or SNP analysis
Unit Definition
One activity unit is the amount of enzyme required to catalyze the incorporation reaction of 10 nmol dNTP into acid insoluble substances in 30 minutes at 74°C.
Storage
Stored at -20°C.
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