Cat. No.: BSD-8k (for 8KU)

Cat. No.: BSD-40k (for 40KU)

For isothermal amplification of RNA, please see Bst DNA/RNA Polymerase.

For custom-ready mastermix (including visualization dye, probes, primers, etc), please email your requirements to tech@sbsbio.com.

The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.

Description

Bst DNA Polymerase is a homologous protein of Bst DNA Polymerase, large fragment, derived from the thermophilic bacterium Bacillus stearothermophilus (Bst). When compared to the large fragment of Bst DNA Polymerase, it exhibits stronger 5'→3' DNA polymerase activity, enhanced strand displacement capability, tolerance to dUTP, salt resistance, and resistance to non-ionic detergents. Bst DNA Polymerase lacks 5'→3' and 3'→5' exonuclease activity. It can be used in various applications, including loop-mediated isothermal amplification (LAMP), crossing priming amplification (CPA), rolling-circle amplification (RCA), and isothermal amplification reactions based on rolling-circle amplification.

The isothermal amplification temperature mediated by Bst DNA Polymerase generally falls between 50-68°C, typically at 65°C. The optimal temperature depends on the primers and the products being amplified and may require experimental optimization.

Compared to the Bst 2.0 DNA Polymerase from similar companies, this product exhibits similar enzyme activity, comparable high dUTP tolerance, similar high tolerance to non-ionic detergents, and equivalent salt resistance.

Source

Bst DNA Polymerase is obtained through recombinant expression and purification in Escherichia coli.

Purity

Free from DNA endonuclease and exonuclease activities.

Applications

Suitable for various DNA isothermal amplification techniques, including loop-mediated isothermal amplification (LAMP), crossing priming amplification (CPA), rolling-circle amplification (RCA), helicase-dependent amplification (HDA), multiple displacement amplification (MDA), strand displacement DNA synthesis, whole-genome amplification (WGA), high GC-content DNA sequencing, rapid sequencing of nanogram-level DNA templates, library sequencing, and more.

Enzyme Storage Buffer

10mM Tris-HCl, 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol (pH 7.5 @ 25°C).

10X Reaction Buffer

200mM Tris-HCl, 500mM KCl, 100mM (NH4)2S04, 20mM MgS04, 1% Tween-20 (pH 8.8 @ 25°C).

Inactivation or Inhibition

Bst DNA Polymerase can be inactivated by heating at 80°C for 20 minutes.

Storage Conditions

Store the components at -20°C. Avoid multiple freeze-thaw cycles.

Instruction: Protocol

Related: 

• Bst DNA Polymerase Large Fragment
• Bst DNA Polymerase (comparable to 2.0)
• Bst Polymerase (comparable to 3.0)
• Bst Polymerase (glycerol-free)
• Bst DNA/RNA Polymerase (with superior reverse transcription activity) 
• Bst DNA/RNA Polymerase (glycerol-free)
• Bst P DNA/RNA Polymerase (glycerol-free) (with superior reverse transcription activity & hot-start property) 

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Only for research and not intended for treatment of humans or animals