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RNase A (DNase and Protease-free)
$87.50 - $700.00
$1,000.00
RNase A, without DNase and protease, is an endoribonuclease that specifically degrades the C and U residues of single-stranded RNA. It can cleave the phosphodiester bond between the nucleotide 5'-ribose and the attached phosphate group on the adjacent pyrimidine nucleotide 3'-ribose. The obtained 2', 3'-cyclic phosphate can be further hydrolyzed to the corresponding 3'-nucleoside phosphate.
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Cat. No.: RNASEA-100 (for 100mg)
Cat. No.: RNASEA-1k (for 1000mg)
General Information
- CAS No.: 9001-99-4
- E.C.: 3.1.27.5
- Source: Recombinant. Produced and purified from yeast cells.
- Concentration: 10 mg/mL
- Activity: ≥ 350Kunitz units/mL
- Molecular weight: 14.3 kDa
- Form: Liquid: clear colorless buffered aqueous glycerol solution.
- Grade： Molecular biology level
- Purity: ≥ 90%; (SDS-PAGE)
- Endotoxin: None detected (Tachypleus Amebocyte Lysate)
- Foreign activity: Endonuclease and exonuclease, none detected. NICKase and DNase, none detected
- Optimal pH: 7.6 (activity range of 6-10)
- Optimal temperature: 60°C (activity range of 15-70°C)
Application
- The removal of RNA from preparations of biological products, such as plasmid DNA or protein samples.
- RNA sequence analysis
- RNase protection assays
Unit Definition
One unit is defined as the amount of RNase A that causes an increase of absorbance by 1.0 unit at 260 nm when yeast RNA is hydrolyzed at 37°C and pH 5.0.
Storage
The minimum shelf life is one year at -20°C.
- Storage buffer: 10 mM Tris-HCl (pH 8.0), 50% glycerol.
- Dilution buffer: 10 mM Tris-HCl (pH 8.0).
Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.
Only for research and not intended for treatment of humans or animals
