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Cat. No.: XRN1-20 (for 20U)

Cat. No.: XRN1-100 (for 100U)

Cat. No.: XRN1-500 (for 500U)

Description

XRN-1, also known as 5’-Phosphate Dependent Exonuclease, is a highly active 5’-3’ exoribonuclease expressed and purified from Escherichia coli. It requires the substrate RNA to have a 5’ monophosphate and catalyzes the degradation of 5’ monophosphate RNA (p-RNA) into NMP in the 5’→3’ direction. XRN-1 can also digest single-stranded DNA (ssDNA) with a 5’ monophosphate, though the cleavage efficiency is lower. XRN-1 cannot digest RNA with 5’ triphosphate (5’-ppp), 5’ diphosphate (5’-pp), 5’ cap, or 5’ hydroxyl (5’-OH), as well as double-stranded DNA (dsDNA) or ssDNA with 5’ hydroxyl, triphosphate, or diphosphate. XRN-1 also cannot digest 5’ phosphate-ended recessed double-stranded RNA, DNA-RNA hybrids, or RNA in secondary structures. XRN-1 activity requires Mg²⁺, and the catalytic reaction is as follows:

p-RNA → NMP

The enzymatic properties of XRN-1 make it useful for selectively digesting ribosomal RNA (rRNA) to isolate and enrich messenger RNA (mRNA) from total RNA without relying on oligo-dT magnetic beads or agarose gels, facilitating the construction of mRNA sequencing libraries. XRN-1 can also be used for 5’ end RNA analysis.

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Source

Expressed in Escherichia coli, with the gene encoding XRN-1 from yeast.

Molecular Weight

Approximately 175 kDa.

Activity Definition

One unit digests 1 µg of rRNA substrate into acid-soluble nucleotides in 60 minutes at 30ºC under standard assay conditions.

Purity

Free from RNAse and DNA endonucleases and exonucleases other than its own activity.

Precautions

• During use, it is recommended to keep the product in an ice box or on an ice bath. After use, it should be immediately stored at -20°C.
• This product is intended for scientific research purposes by professionals only. It is not intended for clinical diagnosis or treatment, and should not be used for food or drug purposes. It should not be stored in a regular household setting.
• For your safety and health, please wear lab attire and disposable gloves when handling the product.

Storage

The minimum shelf life is 2 year at -20°C.

Related: RNA 5’ Pyrophosphohydrolase (RppH)