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We are proud to introduce our groundbreaking RPA Multiplex Fluorescent Lyophilized Kit (Gen-4). This revolutionary exclusive multiplex Recombinase Polymerase Amplification (RPA) kit is capable of completing direct amplification of DNA/RNA within just 10 to 20 minutes.

Cat. No.: RIB3-48 (for 48T)

Cat. No.: RIB3-480 (for 480T)

Description

This product is the Gen-3 version of RPA (Recombinase Polymerase Amplification) nucleic acid amplification reagents, featuring the additional introduction of the dUTP-UDG-UGI system to effectively prevent aerosol contamination. Supplied as a ready-to-use lyophilized reagent, it includes all components except primers, probes, Mg²⁺, and the template.

To use, simply dissolve the RPA lyophilized particles in nucleic acid release solution, add the template, primers, and Mg²⁺. The final reaction volume is 25 µL. Under constant temperature conditions of 39 to 42°C, the system specifically recognizes and amplifies nucleic acid fragments of the target sample. Detection and assessment can be performed in conjunction with gel electrophoresis or real-time fluorescence method.

Ideal for rapid DNA amplification and detection.

Composition

Recombinase, Polymerase, ATP, dUTP, dATP, dCTP, dGTP, sucrose-sucrose phosphorylase, UGI, buffer, poly A, MgOAc.

Nucleic acid release solution (Optional): UDG, Tris-OAc, Triton   X-100, EDTA, AFSE-15, TET

Features

• First introduction of the dUTP-UDG contamination prevention system in RPA
• Effectively removes aerosol contamination at 10⁶ copies/mL
• A perfect companion for POCT and CRISPR

Storage

This product is transported and stored at 2~28°C, in a dry, and light-protected environment, with a shelf life of 1 year.

Related:

• Gen-1: RPA Basic Kit (Gen-1), RPA RT Basic Kit (Gen-1), RPA Fluorescent Kit (Gen-1), RPA RT Fluorescent Kit (Gen-1)
• Gen-2: RPA Basic Kit (Gen-2), RPA RT Basic Kit (Gen-2), RPA Fluorescent Kit (Gen-2), RPA RT Fluorescent Kit (Gen-2)
• Gen-3: RPA Basic Kit (Gen-3), RPA RT Basic Kit (Gen-3), RPA Fluorescent Kit (Gen-3), RPA RT Fluorescent Kit (Gen-3)
• Gen-4: RPA Multiplex Fluorescent Lyophilized Kit (Gen-4)

Only for research and not intended for treatment of humans or animals