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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: RHIII-200 (for 200U)

Cat. No.: RHIII-1k (for 1000U)

Description

RNase HIII is an endoribonuclease that cleaves the phosphodiester bonds at the 5' end of RNA bases in a DNA double-strand, similar to RNase HII. This cleavage results in a nick, generating a 5' phosphate and 3' hydroxyl end. RNase HII, however, cleaves the RNA portion along the Okazaki fragment at multiple sites (i.e., RNase H activity). The enzyme does not hydrolyze pure DNA strands or single-stranded RNA. RNase HIII prefers Mn2+ ions, while RNase HII prefers Mg2+ ions. Our RNase HIII is recombinantly expressed and purified through multiple steps.

Activity Definition

The enzyme quantity required to cleave 100 pmol of double-stranded DNA substrate containing a single ribonucleotide at 37°C in 1× AM Buffer E within 30 minutes.

Activity Assay Conditions

AM Buffer E, incubation at 37°C.

Features

• Prefers Mn2+ ions.
• Possesses RNase H activity simultaneously.

Applications

• Cleaving double-stranded DNA containing ribonucleotides.
• Generating double-strand breaks at sites of inserted ribonucleotides when used in conjunction with T7 Endo I.
• Degradation of the RNA portion of Okazaki fragments.

Storage

Store at -20°C.

Related:

• RNase H
• Thermostable RNase H
• Tth Thermostable RNase H
• Tth Thermostable RNase HII
• Extreme Thermostable RNase HII
• RNase HIII

Only for research and not intended for treatment of humans or animals

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